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Biocontained Carcass Composting for Control of Infectious Disease Outbreak in Livestock
Published on: May 6, 2010
Thermomonospora catenispora sp. nov., isolated from mushroom compost
Hao Wu1,2, Jinfan Wei1, Bin Liu1
1Institute of Applied Microbiology, College of Agriculture, Guangxi University, Nanning, PR China.
Abstract:
A novel thermophilic actinomycete, designated strain 3-22-3T, was isolated from mushroom compost collected in Nanning, Guangxi Province, China. The organism produced white aerial mycelium and short spore chains of non-motile oval spores with a ridged surface on the aerial mycelium. Strain 3-22-3T contained meso-diaminopimelic acid as the diagnostic diamino acid. The whole-cell sugars were galactose, glucose, madurose and ribose. Major fatty acids were iso-C17 : 0, iso-C16 : 0, iso-C15 : 0 and anteiso-C17 : 0. MK-9(H6) and MK-9(H4) were the predominant menaquinones. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, ninhydrin-positive glycophospholipids, glycolipids and unidentified phospholipids. The G+C content of the genomic DNA was 72.5 mol%. The 16S rRNA gene sequence analysis indicated that strain 3-22-3T belonged to the genus Thermomonospora and showed the highest sequence similarity to Thermomonospora curvata DSM 43183T (96.3 %). On the 16S rRNA gene tree of closely related species and type species of all genera in the family Thermomonosporaceae, strain 3-22-3T formed a distinct phyletic line together with Thermomonospora curvata DSM 43183T. Furthermore, the chemotaxonomic characteristics of strain 3-22-3T were congruent with the description of the genus Thermomonospora, but the morphological characteristics of strain 3-22-3T were significantly different from Thermomonosporacurvata. Based on the phenotypic and phylogenetic data, strain 3-22-3T represents a novel species of the genus Thermomonospora, and the name Thermomonospora catenispora sp. nov. is proposed. The type strain is 3-22-3T (=CGMCC 4.7155T=DSM 46802T=ATCC BAA-2627T).
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