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Related Experiment Videos

Mitogen-activated B-cell blasts reactive to more than one mitogen.

J Andersson, A Coutinho, F Melchers

    The Journal of Experimental Medicine
    |March 1, 1979
    PubMed
    Summary

    Most splenic B cells can respond to multiple mitogens, indicating they possess multi-reactive receptors. These receptors regulate B cell growth and IgM secretion, crucial for immune responses.

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    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Splenic B cells play a critical role in adaptive immunity.
    • Activation by mitogens like lipopolysaccharide (LPS) and lipoprotein (LP) drives B cell growth and differentiation into antibody-secreting cells.

    Purpose of the Study:

    • To investigate the mitogenic reactivity of splenic B cells.
    • To determine if B cells can respond to multiple types of mitogens.
    • To explore the implications for B cell receptor complexes.

    Main Methods:

    • Activation of splenic B cells from C57BL/6J and C3H/Tif mice with LPS or LP.
    • Separation of activated blast cells via velocity sedimentation.
    • Restimulation assays with homologous and heterologous mitogens.
    • Frequency analysis of reactive B cells.

    Main Results:

    • Activated B cells continue to secrete IgM even without mitogen re-addition.
    • Restimulation with homologous mitogens leads to sustained proliferation and IgM secretion.
    • A significant proportion of B cells respond to multiple mitogens, including LPS, LP, Nocardia mitogen, and fetal calf serum components.
    • LPS-nonresponder C3H/HeJ B cells activated by LP can be restimulated by other mitogens but not LPS.

    Conclusions:

    • The majority of splenic B cells exhibit reactivity to more than one B cell mitogen.
    • B cells possess multi-reactive mitogen-receptor complexes on their surface.
    • These complexes regulate B cell growth and maturation, highlighting the complex nature of B cell activation pathways.

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