The Balance between Actin-Bundling Factors Controls Actin Architecture in Pollen Tubes

How actin-bundling factors cooperatively regulate shank-localized actin bundles remains largely unexplored. Here we demonstrate that FIM5 and PLIM2a/PLIM2b decorate shank-localized actin bundles and that loss of function of PLIM2a and/or PLIM2b suppresses phenotypes associated with fim5 mutants. Specifically, knockout of PLIM2a and/or PLIM2b partially suppresses the disorganized actin bundle and intracellular trafficking phenotype in fim5 pollen tubes. PLIM2a/PLIM2b generates thick but loosely packed actin bundles, whereas FIM5 generates thin but tight actin bundles that tend to be cross-linked into networks in vitro. Furthermore, PLIM2a/PLIM2b and FIM5 compete for binding to actin filaments in vitro, and PLIM2a/PLIM2b decorate disorganized actin bundles in fim5 pollen tubes. These data together suggest that the disorganized actin bundles in fim5 mutants are at least partially due to gain of function of PLIM2a/PLIM2b. Our data suggest that the balance between FIM5 and PLIM2a/PLIM2b is crucial for the normal bundling and organization of shank-localized actin bundles in pollen tubes.