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Related Experiment Video

Updated: Jan 23, 2026

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index
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Does conventional freezing affect sperm DNA fragmentation?

Minh Tam Le1,2, Thai Thanh Thi Nguyen2, Tung Thanh Nguyen3

  • 1Department of Obstetrics and Gynecology, Hue University of Medicine and Pharmacy, Hue University, Hue, Vietnam.

Clinical and Experimental Reproductive Medicine
|June 12, 2019
PubMed
Summary
This summary is machine-generated.

Conventional freezing significantly reduces sperm motility and viability, and increases sperm DNA fragmentation. This cryopreservation method impacts sperm DNA integrity, a crucial factor in assisted reproductive technology success.

Keywords:
CryopreservationDNA fragmentationFreezingHalosperm testSpermatozoa

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Area of Science:

  • Reproductive Biology
  • Andrology
  • Sperm Cryopreservation

Background:

  • Sperm cryopreservation is vital for assisted reproductive technology and treating male infertility.
  • Cryopreservation can negatively affect sperm membrane integrity, acrosome status, motility, and viability.
  • Evaluating DNA damage post-cryopreservation is essential for understanding its impact.

Purpose of the Study:

  • To assess the DNA fragmentation damage in human sperm after conventional freezing.
  • To evaluate the effects of cryopreservation on sperm parameters and DNA integrity using the sperm chromatin dispersion test.

Main Methods:

  • 120 fresh human semen samples were cryopreserved using conventional freezing with SpermFreeze Solution.
  • Semen analysis and Halosperm test (sperm DNA fragmentation) were conducted pre-freezing and post-thawing.
  • Comparison of semen parameters and DNA fragmentation index (DFI) before and after cryopreservation.

Main Results:

  • Significant reductions observed in sperm progressive motility (32.78% to 16%) and viability (79.58% to 55.99%) post-freezing.
  • Sperm DNA fragmentation index (DFI) increased significantly from 19.21% pre-freezing to 22.23% post-thawing.
  • Increased DFI was noted across samples with varying initial motility, morphology, and viability.

Conclusions:

  • Conventional sperm freezing negatively impacts sperm parameters, including motility and viability.
  • Cryopreservation using conventional methods demonstrably reduces sperm DNA integrity.
  • The findings highlight the potential risks of DNA damage associated with sperm cryopreservation.