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Proteomics of Bordetella pertussis whole-cell and acellular vaccines

Jens Möller1, Max Edmund Kraner2, Andreas Burkovski3

  • 1Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr. 5, 91058, Erlangen, Germany.

BMC Research Notes
|June 12, 2019
PubMed

Insights

This study analyzed whole-cell and acellular pertussis vaccines using mass spectrometry. Researchers identified proteins in these vaccines, contributing to understanding host-pathogen interactions in whooping cough.

Area of Science:

  • Microbiology
  • Immunology
  • Biochemistry

Background:

  • Whooping cough, caused by Bordetella pertussis, is a serious bacterial infection, particularly in children.
  • Vaccination strategies are crucial for managing pertussis, but their impact on host-pathogen interactions requires further investigation.
  • Understanding the protein composition of pertussis vaccines is key to evaluating their efficacy and potential effects.

Purpose of the Study:

  • To investigate the protein content of whole-cell and acellular pertussis vaccines.
  • To analyze the host-pathogen interaction and clonal distribution of Bordetella pertussis strains.
  • To identify proteins within pertussis vaccines using mass spectrometry.

Main Methods:

  • Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was employed to analyze vaccine samples.
  • Bordetella pertussis genome data was utilized for protein identification.
  • Bioinformatic tools were used to determine protein subcellular localization and the presence of signal peptides.

Main Results:

  • Proteins present in both whole-cell and acellular pertussis vaccines were identified.
  • Analysis provided insights into the molecular components of pertussis vaccines.
  • Bioinformatic analysis characterized the identified proteins.

Conclusions:

  • The study successfully identified proteins in whole-cell and acellular pertussis vaccines.
  • This proteomic analysis contributes to a deeper understanding of vaccine composition.
  • Findings support further research into vaccine-induced host-pathogen interactions.
Abstract

Keywords:
BordetellaDTP vaccineLC–MS/MSMass spectrometryPertussisVaccinationWhooping cough

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