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Related Experiment Videos

Changes in glomerular cross-sectional area induced by platelet activating factor.

G de Arriba1, V Barrio, L Hernando

  • 1Department of Nephrology, Fundación Jiménez Díaz, Madrid, Spain.

Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
|January 1, 1987
PubMed
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Platelet-activating factor (PAF) reduces glomerular size in rats, impacting kidney filtration. This effect is blocked by specific inhibitors, suggesting a role for calcium signaling in PAF

Area of Science:

  • Nephrology
  • Renal Physiology
  • Pharmacology

Background:

  • Platelet-activating factor (PAF) is a potent lipid mediator implicated in various physiological and pathological processes.
  • Its role in directly affecting glomerular structure and function, independent of hemodynamic changes, requires further elucidation.

Purpose of the Study:

  • To investigate the direct effect of platelet-activating factor (PAF) on glomerular morphology and its underlying mechanisms.
  • To determine if PAF influences glomerular filtration coefficient (Kf) by altering the filtration surface area.

Main Methods:

  • Isolated rat glomeruli were incubated with varying concentrations and durations of PAF.
  • Glomerular cross-sectional area was measured using computerized image analysis.
  • The effects of PAF were tested in the presence of inhibitors (BN-52021, verapamil, acetylsalicylate of lysine) and calcium-depleted media.

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Main Results:

  • PAF induced a significant, time-dependent reduction in glomerular cross-sectional area starting at 10(-8) M.
  • This reduction was inhibited by BN-52021, verapamil, and calcium-free media, but not by acetylsalicylate of lysine.
  • Results indicate PAF's action is mediated via specific receptors and intracellular calcium changes, independent of cyclooxygenase metabolites.

Conclusions:

  • Platelet-activating factor directly reduces glomerular filtration surface area.
  • This effect is mediated by PAF receptor interaction and subsequent intracellular calcium modulation.
  • PAF may influence glomerular filtration rate (GFR) by altering the filtration surface, thus impacting Kf.