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Characterization of uraemia-induced decrease in insulin binding on erythrocytes.

A Marttinen1, A Pasternack, T Koivula

  • 1Department of Clinical Sciences, University of Tampere, Finland.

Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
|January 1, 1987
PubMed
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Uremic plasma contains inhibitors that impair insulin binding to erythrocyte insulin receptors, contributing to insulin insensitivity in uremia. Hemodialysis partially reduces this inhibitory effect.

Area of Science:

  • Endocrinology
  • Nephrology
  • Biochemistry

Background:

  • Uremia, a condition associated with kidney failure, is linked to metabolic disturbances.
  • Insulin resistance is a common complication in patients with uremia.
  • The precise mechanisms underlying insulin resistance in uremia require further elucidation.

Purpose of the Study:

  • To investigate the presence and effect of inhibitors in uremic plasma on insulin binding to erythrocyte insulin receptors.
  • To determine if uremic erythrocytes have intrinsic defects in their insulin receptors.
  • To assess the impact of hemodialysis on the inhibitory capacity of uremic plasma.

Main Methods:

  • Insulin radioreceptor assays were performed using healthy and uremic erythrocytes.
  • Uremic plasma was incubated with healthy erythrocytes at varying insulin concentrations.

Related Experiment Videos

  • Insulin binding was quantified in the presence and absence of uremic plasma before and after hemodialysis.

    • Main Results:

    • Uremic plasma demonstrated significant inhibition of insulin binding to healthy erythrocyte insulin receptors.
    • No intrinsic defects in insulin receptors of uremic erythrocytes were observed.
    • The inhibitory effect of uremic plasma on insulin binding was reduced after hemodialysis treatment.

    Conclusions:

    • Uremic plasma contains inhibitors that directly impair insulin receptor function.
    • These plasma-borne inhibitors contribute to insulin insensitivity observed in uremia.
    • Hemodialysis offers a partial reversal of this insulin receptor inhibition.