Related Experiment Video

Updated: Jan 23, 2026

Visualizing DNA Damage Repair Proteins in Patient-Derived Ovarian Cancer Organoids via Immunofluorescence Assays
04:07

Visualizing DNA Damage Repair Proteins in Patient-Derived Ovarian Cancer Organoids via Immunofluorescence Assays

Published on: February 24, 2023

2.2K

The Functions of DNA Damage Factor RNF8 in the Pathogenesis and Progression of Cancer

Tingting Zhou1, Fei Yi1, Zhuo Wang1

  • 1Institute of Translational Medicine, China Medical University; Key Laboratory of Medical Cell Biology, Ministry of Education; Liaoning Province Collaborative Innovation Center of Aging Related Disease Diagnosis and Treatment and Prevention, Shenyang, Liaoning Province, China.

International Journal of Biological Sciences
|June 12, 2019
PubMed

Insights

Really Interesting New Gene (RING) finger protein 8 (RNF8) is crucial for DNA repair and genomic stability. Understanding RNF8

Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • DNA double-strand breaks (DSB) represent the most cytotoxic DNA damage, leading to genomic instability.
  • The Really Interesting New Gene (RING) finger protein 8 (RNF8) is a key mediator in the cellular response to DSBs.
  • RNF8 plays critical roles beyond DNA damage response, including telomere protection, cell cycle control, and transcriptional regulation.

Purpose of the Study:

  • To review the diverse functions of RNF8 in DNA damage response and other cellular processes.
  • To elucidate the mechanisms underlying RNF8's involvement in tumorigenesis and cancer progression.
  • To explore the therapeutic potential of targeting RNF8 for cancer treatment strategies.

Main Methods:

  • Literature review of studies on RNF8.
  • Analysis of RNF8's role in DNA damage signaling pathways.
  • Examination of RNF8's involvement in cancer pathogenesis through various experimental models.

Main Results:

  • RNF8 is essential for efficient DNA double-strand break repair and maintaining genomic integrity.
  • RNF8's functions in DNA repair, telomere maintenance, and cell cycle control are intrinsically linked to cancer development.
  • RNF8 deficiency in a mouse model resulted in spontaneous tumor formation, highlighting its tumor-suppressive role.

Conclusions:

  • RNF8 is a multifunctional protein with critical roles in DNA repair and cellular homeostasis.
  • Dysregulation of RNF8 contributes significantly to tumorigenesis and cancer progression.
  • Targeting RNF8 pathways presents a promising avenue for novel cancer therapies.
Keywords:
DSBRNF8cell cycletelomeretranscriptional regulationtumor

More Related Videos

Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay10:00

Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay

Published on: December 19, 2012

28.1K
Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells05:18

Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells

Published on: June 9, 2020

11.8K

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.7K
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
3.1K
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
10.0K
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
82.3K
Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
33.5K
Asthma: Pathogenesis and Management01:20

Asthma: Pathogenesis and Management

Asthma is a chronic pulmonary condition involving inflammation of the airways, hyper-reactivity, and reversible obstruction of the airways. This condition can significantly impact a person's quality of life, making breathing difficult and leading to distressing symptoms.
Asthma is classified as allergic and non-allergic. Allergens such as dust mites, pollen, and pet dander trigger allergic asthma, while factors like cold air, intense emotions, or exercise can induce non-allergic asthma.
1.3K