Nano based lutein extraction from marigold petals: optimization using different surfactants and co-surfactants

Nanotechnology has high potential in processing of industrial crops and by-products in order to extract valuable biological active compounds. The present study endeavored to take advantage of nanotech approach (i.e microemulsion, ME), as a novel green technique, for lutein extraction from marigold (Tagetes erecta) as an industrial crop. The pseudo-ternary phase diagrams confirmed the effect of surfactant type on the formation of mono-phasic lutein MEs. The combination of sucrose monopalmitate:1-poropanol (1:5) showed the highest efficiency in the presence of marigold petal powder (MPP, 18%) and water (42%). In addition, the efficiency of primitive MEs (without co-surfactants) was outstandingly increased as MPP was moistened by co-surfactants. Furthermore, different MEs resulted in various droplet size (14-250nm), PDI (0.05-0.32) and zeta potential (-1.96 to -38.50 mV). These findings revealed the outstanding importance of the surfactants and co-surfactants and their ratio on the extraction capability of MEs. These findings also proved the capability of microemulsion technique (MET) as a potential alternative to conventional solvent with possible applicability for extraction of lutein and other industrial plant based bio-compounds.