Related Experiment Video
Updated: Jan 23, 2026

3D Kinematic Analysis for the Functional Evaluation in the Rat Model of Sciatic Nerve Crush Injury
Published on: February 12, 2020
Functional analysis of the exocyst subunit BcExo70 in Botrytis cinerea
Wenqing Guan1, Juan Feng2, Rongxia Wang1
1College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, Zhejiang, China.
Abstract:
Botrytis cinerea is one of the most important saprophytic plant pathogenic fungi. The exocyst complex and exocytosis was demonstrated to be involved in fungal development and plant infection. Here, we investigated the function of an exocyst subunit gene Bcexo70 in B. cinerea. The results show that knockout of the Bcexo70 gene significantly reduced the fungal growth and hindered the production of conidia and sclerotia. The Bcexo70 deletion strains showed a severe decrease in virulence toward tomato leaves and reduced secretion of cell wall-degrading enzyme. Confocal and electronic microscopic observation showed that the vesicles in the Bcexo70 mutants were enlarged and scattered in the cytoplasm compared to the regular distribution in the hyphal tip in wild-type strain. This study showed that the exocyst gene Bcexo70 is crucial for fungal growth, conidiation and pathogenicity in B. cinerea.
More Related Videos
22:10Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit
Published on: June 28, 2013
09:36Recombinant α- β- and γ-Synucleins Stimulate Protein Phosphatase 2A Catalytic Subunit Activity in Cell Free Assays
Published on: August 13, 2017
Related Concept Videos
Performing a Simple Data Analysis using MS-Excel Function
SUM: This function calculates the total sum of a range of values. It's the foundation for aggregating data, essential for determining overall trends and totals in datasets.
AVERAGE: It computes the mean value of a given set of numbers, providing a quick insight into the central...
Functional Groups
Functional Groups
Functionalism
James envisioned psychology's...
Mechanical Protein Functions
Structural Protein Function
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...