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Fibrinolytic activity in medium from tissue culture of paranasal mucous membrane
1Dept. of Physiology, School of Medicine, University of the Ryukus, Okinawa, Japan.
Abstract:
It is known that a remarkable fibrinolytic activity of plasminogen activator (PA) can be seen in extracts of wet tissue and acetone powder preparation of paranasal mucous membrane evidencing chronic sinusitis. However, the origin of the PA in extract of paranasal mucous membrane has not yet been clarified up to the present time. In this experiment, using a tissue culture of paranasal mucous membrane, it was observed that two species of cells, epithelial cells and fibrocytes, proliferated in the implanted tissue. PA was isolated from the conditioned medium on the fifth day after culture. From these results, it appears that the PA may be released from epithelial cells and/or fibroblasts.
Insights
Researchers identified the source of plasminogen activator (PA) in chronic sinusitis tissues. PA, crucial for fibrinolysis, is released by epithelial cells and fibrocytes in the paranasal mucous membrane.
Area of Science:
- Biomedical Research
- Cell Biology
- Biochemistry
Background:
- Chronic sinusitis is associated with significant fibrinolytic activity in paranasal mucous membrane extracts.
- The cellular origin of plasminogen activator (PA), responsible for this activity, remains unclear.
Purpose of the Study:
- To investigate the cellular source of plasminogen activator (PA) in paranasal mucous membrane tissue.
Main Methods:
- Tissue culture of paranasal mucous membrane.
- Isolation and identification of PA from conditioned cell culture medium.
Main Results:
- Epithelial cells and fibrocytes were observed to proliferate in the cultured paranasal mucous membrane tissue.
- Plasminogen activator (PA) was successfully isolated from the conditioned medium on the fifth day of culture.
Conclusions:
- The findings suggest that epithelial cells and/or fibrocytes are the source of plasminogen activator (PA) in paranasal mucous membrane.
- This clarifies the origin of PA contributing to fibrinolytic activity in chronic sinusitis.