Induction of IL-2 and lymphokine activated killer cells in the cat

M B Tompkins¹, G K Ogilvie, R A Franklin

¹Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana 61801.

Veterinary Immunology and Immunopathology

|September 1, 1987

Abstract:

We have described the use of a cloned murine IL-2-dependent T-cell line to directly measure feline IL-2. Concanavalin A stimulated feline peripheral blood lymphocytes produced an IL-2-rich supernatant that supported the growth of this murine IL-2-dependent T-cell line. In addition to producing IL-2, Con A stimulated killer cells in PBL were cytotoxic for the FeLV transformed tumor cell line FL74. Incubating feline PBL with a cocktail of the calcium ionophore A23187 and phorbol ester also led to the generation of cytotoxic cells as well as the production of high levels of IL-2. Finally, IL-2-rich supernatant was able to stimulate cytotoxic activity in PBL from normal cats.

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Abstract:

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