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Rapid single B cell antibody discovery using nanopens and structured light
Aaron Winters1, Karyn McFadden1, John Bergen2
1a Department of Therapeutic Discovery, Amgen Research , Thousand Oaks , CA , USA.
Mabs
|June 13, 2019
Summary
We developed NanOBlast, a rapid single B cell screening method for generating therapeutic antibody reagents. This technique accelerates the discovery of high-affinity anti-idiotypic monoclonal antibodies for drug development.
Area of Science:
- Biotechnology
- Immunology
- Drug Development
Background:
- Accelerated development of monoclonal antibody (mAb) tool reagents is crucial for therapeutic antibody advancement.
- The drug development market demands faster and more efficient methods for generating essential reagents.
Purpose of the Study:
- To describe a novel nanofluidic optoelectronic single B lymphocyte antibody screening technique (NanOBlast).
- To apply NanOBlast for the rapid generation of anti-idiotypic reagent antibodies.
- To develop a sensitive assay for quantifying therapeutic IgG molecules in patient serum.
Main Methods:
- Utilized NanOBlast, a high-content culture and assay platform, to screen single antibody secreting cells (ASCs).
- Employed microfluidic chip nanopens and structured light for cell manipulation and screening.
- Integrated single-cell PCR, recombinant IgG expression, and ELISA for antibody characterization.
Main Results:
- Successfully recovered and identified a diverse panel of high-affinity anti-idiotypic reagent mAbs.
- Developed a sensitive and specific ligand binding assay for quantifying free therapeutic IgG in human serum.
- Achieved a 5-hour workflow for cell screening on-chip and under 60 days for the overall discovery process.
Conclusions:
- NanOBlast offers a direct, flexible, and rapid method for generating critical mAb tool reagents.
- The developed assay facilitates crucial drug development decision-making by enabling precise therapeutic IgG quantification.
- This technology significantly accelerates the timeline for therapeutic antibody reagent discovery and development.
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