Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹H NMR Signal Multiplicity: Splitting Patterns01:13

¹H NMR Signal Multiplicity: Splitting Patterns

6.5K
When protons A and X are coupled, their nuclear spin energy levels are slightly modified. This is because the energy required to excite proton A to a spin state parallel to proton X is slightly different from the energy required for it to become anti-parallel to spin X. Consequently, there are two possible excitation frequencies for A (A1 and A2), depending on the spin state of X, and vice versa. The mutual nature of coupling implies that the difference between frequencies A1 and A2, indicated...
6.5K
Types of Stressors01:23

Types of Stressors

666
A stressor is any event, condition, or stimulus that triggers stress and causes a physical or psychological response in the body. Stressors can be categorized into three main types: catastrophes; significant life changes; and daily hassles, including social stress. Each can be detrimental to physical and mental well-being.
Catastrophes
Catastrophes refer to large-scale, unpredictable events that create overwhelming stress and a sense of threat. Examples include natural disasters like...
666
Stream Function01:20

Stream Function

2.1K
In two-dimensional incompressible fluid flow, the continuity equation is essential for ensuring mass conservation, meaning that any change in fluid entering or exiting a region is balanced by a corresponding change elsewhere. For incompressible flow, where density remains constant, this requirement simplifies to the condition that the divergence of the velocity field must be zero. Mathematically, this is expressed as,
2.1K
Steady Flow of a Fluid Stream01:27

Steady Flow of a Fluid Stream

688
Consider a control volume, such as a pipe with solid boundaries, through which fluid flows and changes direction due to the impulse exerted by the resulting force from the pipe walls. In steady flow, the mass of fluid entering the control volume at a given time, t, with velocity v1, is equal to the mass leaving after infinitesimal time dt, with velocity v2.
During this process, the momentum of the fluid within the control volume remains constant over the time interval dt. By applying the...
688
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

25.8K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
25.8K
Fixed Action Patterns01:06

Fixed Action Patterns

17.6K
A fixed action pattern (FAP) is a specific, hard-wired sequence of behaviors that occurs in response to an external stimulus, called a sign stimulus. The behavior is “fixed” because it is essentially unchangeable—proceeding similarly across individuals of a species every time it occurs.
17.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Compounding heterochrony shapes the salamander visual system across adaptive zones.

Proceedings. Biological sciences·2025
Same author

Seasonal heterochrony of reproductive development and gene expression in a polymorphic salamander.

Developmental dynamics : an official publication of the American Association of Anatomists·2024
Same author

Paedomorphic salamanders are larval in form and patterns of limb emergence inform life cycle evolution.

Developmental dynamics : an official publication of the American Association of Anatomists·2022
Same author

Pheromone Gene Diversification and the Evolution of Courtship Glands in Plethodontid Salamanders.

Journal of molecular evolution·2021
Same author

Repeated ecological and life cycle transitions make salamanders an ideal model for evolution and development.

Developmental dynamics : an official publication of the American Association of Anatomists·2021
Same author

Sociality sculpts similar patterns of molecular evolution in two independently evolved lineages of eusocial bees.

Communications biology·2021

Related Experiment Video

Updated: Jan 23, 2026

Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells
08:09

Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells

Published on: August 29, 2022

8.5K

Multiple stressors produce differential transcriptomic patterns in a stream-dwelling salamander.

Timothy A Clay1,2, Michael A Steffen3,4, Michael L Treglia3,5

  • 1Department of Biological Science, University of Tulsa, Tulsa, OK, 74104, USA. tim.clay@nicholls.edu.

BMC Genomics
|June 13, 2019
PubMed
Summary

Transcriptomic signatures reveal stressor-specific patterns in salamander gene expression, offering a novel method for monitoring amphibian health independent of temperature. This approach aids in understanding environmental impacts on wildlife populations.

Keywords:
BiomarkersSalamanderStressTranscriptome

More Related Videos

Isolation of Giant Lampbrush Chromosomes from Living Oocytes of Frogs and Salamanders
10:07

Isolation of Giant Lampbrush Chromosomes from Living Oocytes of Frogs and Salamanders

Published on: December 5, 2016

13.5K
Mining Spatial Transcriptomics Datasets using DeepSpaceDB
10:16

Mining Spatial Transcriptomics Datasets using DeepSpaceDB

Published on: September 5, 2025

680

Related Experiment Videos

Last Updated: Jan 23, 2026

Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells
08:09

Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells

Published on: August 29, 2022

8.5K
Isolation of Giant Lampbrush Chromosomes from Living Oocytes of Frogs and Salamanders
10:07

Isolation of Giant Lampbrush Chromosomes from Living Oocytes of Frogs and Salamanders

Published on: December 5, 2016

13.5K
Mining Spatial Transcriptomics Datasets using DeepSpaceDB
10:16

Mining Spatial Transcriptomics Datasets using DeepSpaceDB

Published on: September 5, 2025

680

Area of Science:

  • Amphibian ecotoxicology
  • Molecular ecology
  • Vertebrate stress physiology

Background:

  • Amphibian populations face a global biodiversity crisis, necessitating effective health monitoring tools.
  • Plasma glucocorticoids are common stress indicators but are difficult to measure in small organisms.
  • Transcriptomic stress responses in amphibians are understudied, especially concerning multiple stressors.

Purpose of the Study:

  • To investigate gene expression patterns in salamander tail tissues under chronic corticosterone exposure and varying temperatures.
  • To identify stressor-specific transcriptional signatures for monitoring amphibian health.
  • To evaluate the consistency of stress-induced gene expression across different life stages and tissues.

Main Methods:

  • Analysis of tail tissue gene expression in stream-dwelling salamanders (Eurycea tynerensis).
  • Exposure to the stress hormone corticosterone under controlled temperature regimes.
  • Evaluation of gene expression patterns across different ages, life cycle modes, and tissue regeneration stages.

Main Results:

  • Identified unique transcriptional signatures for corticosterone exposure, independent of temperature.
  • Discovered corticosterone-responsive genes involved in immune response, oxidative stress, and tissue repair.
  • Observed interactive effects of temperature and corticosterone on specific gene expression patterns, consistent across various life stages.

Conclusions:

  • Transcriptomic patterns provide stressor-specific signatures for monitoring non-human vertebrate health.
  • This approach is underutilized for monitoring diverse stressors in amphibian populations.
  • Gene expression analysis offers a powerful tool for assessing environmental impacts on wildlife health.