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Updated: Jan 23, 2026

Establishment of Viral Infection and Analysis of Host-Virus Interaction in Drosophila Melanogaster
Published on: March 14, 2019
Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection
Annika Schneider1, Sandra Kurz1, Katrin Manske1
1Institute of Molecular Immunology and Experimental Oncology, Klinikum rechts der Isar, Technical University of Munich, Munich, 81567, Germany.
Abstract:
Mitochondria are key for cellular metabolism and signalling processes during viral infection. We report a methodology to analyse mitochondrial properties at the single-organelle level during viral infection using a recombinant adenovirus coding for a mitochondrial tracer protein for tagging and detection by multispectral flow cytometry. Resolution at the level of tagged individual mitochondria revealed changes in mitochondrial size, membrane potential and displayed a fragile phenotype during viral infection of cells. Thus, single-organelle and multi-parameter resolution allows to explore altered energy metabolism and antiviral defence by tagged mitochondria selectively in virus-infected cells and will be instrumental to identify viral immune escape and to develop and monitor novel mitochondrial-targeted therapies.
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