Related Experiment Video

Updated: Jan 23, 2026

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
11:15

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Published on: September 20, 2016

25.0K

Validity of an NGS-based multiple gene panel in identifying actionable mutations for patients with NSCLC in a Chinese

Wei Cao1, Chenghai Yan2, Hailong Wang2

  • 1Department of Cardiothoracic Surgery, The Second Hospital of Anhui Medical University, Hefei, Anhui 230601, P.R. China.

Oncology Letters
|June 13, 2019
PubMed

Insights

A custom next-generation sequencing (NGS) panel identified actionable mutations in 69.6% of non-small cell lung cancer (NSCLC) patients, supporting its use for personalized therapy. This reliable targeted NGS panel aids in discovering gene alterations for tailored cancer treatments.

Area of Science:

  • Oncology
  • Genomics
  • Molecular Diagnostics

Background:

  • Non-small cell lung cancer (NSCLC) is the most prevalent form of lung cancer.
  • Targeted therapies and precision medicine are increasingly important in NSCLC treatment.
  • Multiple-gene panels are utilized in China for precision cancer therapy, necessitating efficiency evaluation.

Purpose of the Study:

  • To evaluate the efficiency of a custom-designed targeted next-generation sequencing (NGS) panel for identifying actionable genomic alterations in NSCLC.
  • To assess the reliability of the FD-180 panel in detecting mutations relevant to targeted therapies and clinical trials.

Main Methods:

  • Investigated 23 NSCLC samples using a custom panel (FD-180) covering ~180 cancer driver genes.
  • Utilized whole exome sequencing on white blood cell samples as controls.
  • Analyzed identified mutations for actionable therapeutic options and clinical trial eligibility.

Main Results:

  • Actionable mutations with available targeted therapies were found in 69.6% of NSCLC cases.
  • Therapeutic targets recommended by National Comprehensive Cancer Network guidelines were identified in 60.9% of cases.
  • Gene mutations qualifying patients for clinical trials or off-label therapies were present in 8.7% of patients.

Conclusions:

  • The custom-designed targeted NGS panel (FD-180) demonstrates reliability for NSCLC.
  • The panel effectively identifies actionable gene alterations for personalized cancer therapy.
  • This approach supports the design of tailored treatment strategies for NSCLC patients.
Keywords:
next generation sequencingnon-small cell lung cancertargeted therapytyrosine kinase inhibitors

More Related Videos

A Strategy to Identify de Novo Mutations in Common Disorders such as Autism and Schizophrenia05:51

A Strategy to Identify de Novo Mutations in Common Disorders such as Autism and Schizophrenia

Published on: June 15, 2011

26.4K
Enema of Traditional Chinese Medicine for Patients with Severe Acute Pancreatitis06:05

Enema of Traditional Chinese Medicine for Patients with Severe Acute Pancreatitis

Published on: January 27, 2023

27.8K

Related Concept Videos

Mutation, Gene Flow, and Genetic Drift01:09

Mutation, Gene Flow, and Genetic Drift

In a population that is not at Hardy-Weinberg equilibrium, the frequency of alleles changes over time. Therefore, any deviations from the five conditions of Hardy-Weinberg equilibrium can alter the genetic variation of a given population. Conditions that change the genetic variability of a population include mutations, natural selection, non-random mating, gene flow, and genetic drift (small population size).
63.3K
Hospitals-II00:59

Hospitals-II

Hospitals provide inpatient and outpatient services. Inpatient services provide care to patients that stay in the hospital for an extended period, ranging from days to months. Examples of inpatient services include intensive care units, hospital wards, or surgeries. Outpatient services provide care to patients who come to a hospital for a diagnostic or treatment but do not stay overnight —for example, diagnostic tests, surgical procedures, or health education.
Nurses that work in...
1.1K
Hospitals-I01:28

Hospitals-I

Hospitals offer medical and surgical care to the sick and injured, along with accommodation while they recover. At the same time, they also provide outpatient, emergency, psychiatric, and rehabilitation services to meet various community needs. In addition to providing medical care, hospitals also act as hubs for medical research and training. Hospitals use clinical procedures and evidence-based practice standards to deliver patient care. To deliver safe and efficient care, a nurse must stay up...
1.5K
Mutations01:39

Mutations

Overview
94.4K
Wood Panel Products01:18

Wood Panel Products

Wood panel products are essential materials used in construction for applications such as flooring, siding, and roofing, typically available in standard dimensions of 4 feet by 8 feet, with thicknesses varying from one-quarter of an inch to one and one-eighth inches. Among the most common types of wood panels is plywood, which is produced by gluing multiple layers of thin wood veneers under pressure. The grain of the outer veneers runs lengthwise, while the grains of the interior layers run...
365
Viral Mutations00:36

Viral Mutations

A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...
39.8K