Tunable microfluidic standing air bubbles and its application in acoustic microstreaming

Microbubbles are often used in chemistry, biophysics, and medicine. Properly controlled microbubbles have been proved beneficial for various applications by previous scientific endeavors. However, there is still a plenty of room for further development of efficient microbubble handling methods. Here, this paper introduces a tunable, stable, and robust microbubble interface handling mechanism, named as microfluidic standing air bubbles (μSABs), by studying the multiphysical phenomena behind the gas-liquid interface formation and variation. A basic μSAB system consists specially structured fluidic channels, pneumatic channels, and selectively permeable porous barriers between them. The μSABs originate inside the crevice structures on the fluidic channel walls in a repeatable and robust manner. The volumetric variation of the μSAB is a multiphysical phenomenon that dominated by the air diffusion between the pneumatic channel and the bubble. Theoretical analysis and experimental data illustrate the coupling processes of the repeatable and linear μSAB volumetric variation when operated under common handling conditions (control pneumatic pressure: -90 kPa to 200 kPa). Furthermore, an adjustable acoustic microstreaming is demonstrated as an application using the alterable μSAB gas-liquid interface. Derived equations and microscopic observations elucidate the mechanism of the continuous and linear regulation of the acoustic microstreaming using varying μSAB gas-liquid interfaces. The μSAB system provides a new tool to handle the flexible and controllable gas-liquid interfaces in a repeatable and robust manner, which makes it a promising candidate for innovative biochemical, biophysical, and medical applications.