Modified methods for measuring citrulline and carbamoyl-beta-alanine with reduced light sensitivity and sucrose...

Two modified procedures for the colorimetric quantitation of citrulline aimed primarily at the assay of ornithine transcarbamylase were developed. Both methods give highly reproducible results in a short period of time since color is developed in 25 min at 100 degrees C. One method is more sensitive than previous methods and the other is as sensitive but requires less than one-fifth as much acid for color development. The reduction in acid concentration results in the stability of the colored complex for at least 50 min under the laboratory lighting conditions and allows for the presence of 5 mumol sucrose and 0.03 mumol mercaptoethanol in the assay. A low-acid modification for quantitating carbamoyl-beta-alanine is described also and may be applicable to the assay of dihydropyrimidinase.