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Chicken apolipoprotein A-I: cDNA sequence, tissue expression and evolution.

L Byrnes1, C C Luo, W H Li

  • 1Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030.

Biochemical and Biophysical Research Communications
|October 14, 1987
PubMed
Summary
This summary is machine-generated.

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Researchers identified chicken apolipoprotein A-I (apoA-I) cDNA, revealing its sequence and expression in various tissues. This finding aids understanding of apoA-I

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Apolipoprotein A-I (apoA-I) plays a crucial role in lipoprotein metabolism.
  • Understanding avian apoA-I is important for comparative studies with mammalian systems.

Purpose of the Study:

  • To identify and characterize the chicken apolipoprotein A-I (apoA-I) cDNA.
  • To determine the nucleotide sequence and predict the protein structure of chicken apoA-I.
  • To investigate the tissue-specific expression of apoA-I mRNA in chickens.

Main Methods:

  • Screening of intestinal cDNA libraries using an antibody against chicken apoA-I.
  • Nucleotide sequencing of identified cDNA clones.
  • Northern blot analysis using a 32P-cDNA probe to detect apoA-I mRNA.

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Main Results:

  • Identification and complete nucleotide sequencing of chicken apoA-I cDNA.
  • Prediction of a mature protein of 240 amino acids, with a 6-amino acid propeptide and an 18-amino acid signal peptide.
  • Detection of apoA-I mRNA in chicken intestine, liver, kidney, spleen, breast muscle, and brain.
  • Observation of tandem repeats in the apoA-I sequence, similar to mammalian proteins.
  • Comparative analysis indicating a faster amino acid substitution rate in rat apoA-I.

Conclusions:

  • The study provides the first complete sequence of chicken apoA-I cDNA.
  • Chicken apoA-I shares structural similarities with mammalian apoA-I, including tandem repeats.
  • ApoA-I is expressed in multiple chicken tissues, suggesting diverse roles.
  • Evolutionary analysis reveals lineage-specific variations in apoA-I sequence evolution.