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Updated: Jan 23, 2026

Experimental Infection of Mice with the Parasitic Nematode Strongyloides ratti ratti Infection
Published on: January 17, 2025
Screening of Strongyloides infection using an ELISA test in transplant candidates
Beatriz Toledo1, Marcelo A Corral1, Dirce Mary C L Meisel1
1Laboratorio de Investigacao Medica (LIM/06 - Laboratorio de Imunopatologia da esquistossomose), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, BR.
Objectives:
Hyperinfection or disseminated strongyloidiasis has been frequently reported after transplants and is related to high mortality. This study aimed to screen for strongyloidiasis using serological diagnoses in transplant candidates.
Methods:
An ELISA test was performed with filariform larvae of Strongyloides venezuelensis as a source of antigen.
Results:
In the serum from transplant candidates, anti-Strongyloides IgG antibodies were detected in 35/150 (23.3%) samples by soluble fractions in phosphate buffered saline (PBS), 31/150 (20.7%) samples by soluble fractions in Tris-HCl, 27/150 (18.0%) samples by membrane fractions in PBS and 22/150 (14.7%) samples by membrane fractions in Tris-HCl.
Conclusions:
The present results suggest the ELISA test, ideally using soluble fractions of filariform larvae S. venezuelensis in PBS, as an additional strategy for the diagnosis of strongyloidiasis in transplant candidates.
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