Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Sense of Self: Reflected Self-Appraisal and Social Comparison02:57

The Sense of Self: Reflected Self-Appraisal and Social Comparison

55.5K
According to Charles Cooley, we base our image on what we think other people see (Cooley 1902). We imagine how we must appear to others, then react to this speculation. We don certain clothes, prepare our hair in a particular manner, wear makeup, use cologne, and the like—all with the notion that our presentation of ourselves is going to affect how others perceive us. We expect a certain reaction, and, if lucky, we get the one we desire and feel good about it. But more than that, Cooley...
55.5K
Renal Drug Clearance: Comparison Between Renal Excretion Methods01:08

Renal Drug Clearance: Comparison Between Renal Excretion Methods

580
Renal clearance is a critical parameter encompassing kidney filtration, secretion, and reabsorption processes. It is calculated using a specific equation to determine the rate at which the kidneys clear a drug.
Renal clearance is often associated with the renal glomerular filtration rate (GFR), which represents the rate at which plasma is filtered through the glomeruli in the kidney. When drug reabsorption is minimal and there is no active secretion, renal clearance is closely related to the...
580
Inertial Frames of Reference01:03

Inertial Frames of Reference

8.7K
Newton’s first law is usually considered to be a statement about reference frames. It provides a method for identifying a special type of reference frame: the inertial reference frame. In principle, we can make the net force on a body zero. If its velocity relative to a given frame is constant, then that frame is said to be inertial. So, by definition, an inertial reference frame is a reference frame where Newton's first law holds valid. Newton's first law applies to objects with...
8.7K
Non-inertial Frames of Reference01:27

Non-inertial Frames of Reference

7.1K
A reference frame accelerating or decelerating relative to an inertial frame is a non-inertial frame. To help understand this, consider what taking off in an airplane, turning a corner in a car, riding a merry-go-round, and the circular motion of a tropical cyclone all have in common. All these systems are accelerating, decelerating, or rotating relative to the Earth; hence, they all are non-inertial frames. All these systems exhibit inertial forces, which merely seem to arise from motion,...
7.1K
Chemical Shift: Internal References and Solvent Effects01:17

Chemical Shift: Internal References and Solvent Effects

1.3K
In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...
1.3K
Multiple Comparison Tests01:13

Multiple Comparison Tests

4.4K
Multiple comparison test, abbreviated as MCT, is a post hoc analysis generally performed after comparing multiple samples with one or more tests. An MCT will help identify a significantly different sample among multiple samples or a factor among multiple factors.
It would be easy to compare two samples using a significance alpha level of 0.05. In other words, there is only one sample pair to be compared. However, it would be difficult to identify a significantly different sample if the number...
4.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A gold nanoparticle/peptide vaccine designed to induce SARS-CoV-2-specific CD8 T cells: a double-blind, randomized, phase 1 study in Switzerland.

BMC infectious diseases·2025
Same author

A Prospective Pilot Study Demonstrating Noninvasive Calibration-Free Glucose Measurement.

Journal of diabetes science and technology·2025
Same author

Randomised, double-blind, controlled phase 1 trial of the candidate tuberculosis vaccine ChAdOx1-85A delivered by aerosol versus intramuscular route.

The Journal of infection·2024
Same author

The Performance of Pulmonary Function Tests in Predicting Systemic Sclerosis-Interstitial Lung Disease in the European Scleroderma Trial and Research Database.

Diagnostics (Basel, Switzerland)·2024
Same author

Safety and immunogenicity of a synthetic nanoparticle-based, T cell priming peptide vaccine against dengue in healthy adults in Switzerland: a double-blind, randomized, vehicle-controlled, phase 1 study.

EBioMedicine·2023
Same author

Nanofluidic point-of-care IgE test for subtropical grass pollen for rapid diagnosis of allergic rhinitis.

Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology·2023

Related Experiment Video

Updated: Jan 23, 2026

Detection of True IgE-expressing Mouse B Lineage Cells
09:40

Detection of True IgE-expressing Mouse B Lineage Cells

Published on: December 1, 2014

11.5K

Novel Nanofluidic IgE Assay versus a Reference Method: A Real-World Comparison.

Samuel Roethlisberger1, Olfa Karoui1, Daria Mapelli1

  • 1Division of Immunology and Allergy, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.

International Archives of Allergy and Immunology
|June 13, 2019
PubMed
Summary

A new nanofluidic assay for specific immunoglobulin E (IgE) testing shows high agreement with a reference method. This point-of-care tool aids in diagnosing allergic diseases by quickly identifying sensitization patterns.

Keywords:
AeroallergenDiagnosisIgENanofluidic technologyPoint-of-care testing

More Related Videos

High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology
08:19

High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology

Published on: May 28, 2020

8.4K
Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
10:22

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

Published on: September 16, 2011

34.1K

Related Experiment Videos

Last Updated: Jan 23, 2026

Detection of True IgE-expressing Mouse B Lineage Cells
09:40

Detection of True IgE-expressing Mouse B Lineage Cells

Published on: December 1, 2014

11.5K
High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology
08:19

High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology

Published on: May 28, 2020

8.4K
Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
10:22

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

Published on: September 16, 2011

34.1K

Area of Science:

  • Allergy Diagnostics
  • Immunology
  • Nanofluidic Technology

Background:

  • Accurate specific immunoglobulin E (IgE) determination is crucial for diagnosing allergic diseases.
  • Current IgE immunoassays often have lengthy turnaround times, creating a clinical need for faster methods.
  • Nanofluidic technology offers potential for rapid, point-of-care allergy diagnostics.

Purpose of the Study:

  • To compare the diagnostic agreement of a novel nanofluidic IgE assay (abioSCOPE®) against a standard laboratory reference method (Phadia Laboratory System®).
  • To evaluate the utility of the nanofluidic assay in a real-world clinical setting for allergy diagnosis.

Main Methods:

  • Sera from 105 patients were analyzed for five respiratory allergens using both the nanofluidic assay and the reference method in a blinded manner.
  • Patient records, including history and skin prick tests, were reviewed by four independent experts to establish final diagnoses.
  • Agreement was assessed by comparing results from the nanofluidic assay and the reference method against expert-determined diagnoses.

Main Results:

  • The nanofluidic IgE assay demonstrated an 81% analytic agreement with the reference method across the tested allergen panel (range: 77-89%).
  • The overall agreement in clinical diagnosis decisions, guided by the nanofluidic assay compared to the reference method, reached 94.6%.

Conclusions:

  • The nanofluidic IgE assay is a valuable tool for allergy experts, enabling point-of-care identification of patient sensitization patterns.
  • This assay supports routine IgE diagnostic workups, integrating clinical history, skin prick tests, and IgE measurements for efficient allergy diagnosis.