Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Biosynthesis of Nucleic Acids01:28

Biosynthesis of Nucleic Acids

1.0K
Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...
1.0K
Criteria for Aromaticity and the Hückel 4n + 2 Rule01:20

Criteria for Aromaticity and the Hückel 4n + 2 Rule

13.0K
Like benzene, cyclobutadiene and cyclooctatetraene are cyclic compounds with alternate single and double bonds. However, their chemical behavior differs from benzene, as they are unstable and not aromatic. So, what are the structural characteristics of unsaturated compounds categorized as aromatic?  
For the first time, Eric Hückel, a German chemical physicist, derived a set of structural features for a compound to be classified as aromatic. This is now known as Hückel’s rule or the 4n +...
13.0K
Acid Halides to Alcohols: LiAlH4 Reduction01:19

Acid Halides to Alcohols: LiAlH4 Reduction

3.9K
Acid halides are reduced to alcohols in the presence of a strong reducing agent like lithium aluminum hydride.
The mechanism proceeds in three steps. First, the nucleophilic hydride ion attacks the carbonyl carbon of the acid halide to form a tetrahedral intermediate. Next, the carbonyl group is re-formed, and the halide ion departs as a leaving group, generating an aldehyde. A second nucleophilic attack by the hydride yields an alkoxide ion, which, upon protonation, gives a primary alcohol as...
3.9K
Biosynthesis in Bacteria01:24

Biosynthesis in Bacteria

593
Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...
593
Biosynthesis of Polysaccharides01:26

Biosynthesis of Polysaccharides

581
Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...
581
Biosynthesis of Lipids01:29

Biosynthesis of Lipids

559
Microbial membranes exhibit remarkable diversity in lipid composition, reflecting evolutionary adaptations to various environmental conditions. The three domains of life—Bacteria, Archaea, and Eukarya—synthesize membrane lipids through distinct biosynthetic pathways, leading to fundamental structural differences that impact membrane stability, function, and adaptability.Fatty Acid-Based Lipids in Bacteria and EukaryaBacteria and eukaryotes share a common fatty acid biosynthesis...
559

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A mechanosensitive lipolytic factor in the bone marrow promotes osteogenesis and lymphopoiesis.

Cell metabolism·2026
Same author

Multilevel Metabolic Engineering of Probiotic <i>Escherichia coli</i> Nissle 1917 for Antibiotic-Free Production of β-Alanine.

ACS synthetic biology·2026
Same author

[Mechanism of moxibustion in inhibiting synovial inflammation of rheumatoid arthritis by regulating macrophage polarization based on the Tim-3/Gal-9 signaling pathway].

Zhen ci yan jiu = Acupuncture research·2026
Same author

A skin-hypothalamus axis couples heat stress and metabolic dysfunction.

Cell·2026
Same author

The Pharmacokinetics and Relative Bioavailability of a Mini-Tablet of Mercaptopurine, a Novel Formulation for Use in Children with Acute Lymphoblastic Leukemia.

Journal of clinical pharmacology·2026
Same author

[Research progress on the mechanism of moxibustion in the treatment of knee osteoarthritis in the past decade].

Zhen ci yan jiu = Acupuncture research·2025

Related Experiment Video

Updated: Jan 23, 2026

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
09:44

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

Published on: March 3, 2015

9.9K

A high-throughput screening method for improved R-2-(4-hydroxyphenoxy)propionic acid biosynthesis.

Hai-Yan Zhou1,2, Yi-Zuo Li1,2, Rui Jiang1,2

  • 1Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, No. 18 Chaowang Road, Hangzhou, 310014, Zhejiang Province, People's Republic of China.

Bioprocess and Biosystems Engineering
|June 14, 2019
PubMed
Summary
This summary is machine-generated.

A new high-throughput screening method enables rapid selection of microorganisms for enhanced biosynthesis of R-2-(4-hydroxyphenoxy)propionic acid (R-HPPA), a key herbicide intermediate. This method identified a Beauveria bassiana mutant with significantly improved R-HPPA production.

Keywords:
High-throughput screeningHydroxylationPotassium dichromateR-2-(4-hydroxyphenoxy)propionic acidR-2-phenoxypropionic acid

More Related Videos

Author Spotlight: High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography
06:19

Author Spotlight: High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

Published on: March 10, 2023

5.6K
Cercosporin-Photocatalyzed [4+1]- and [4+2]-Annulations of Azoalkenes Under Mild Conditions
07:12

Cercosporin-Photocatalyzed [4+1]- and [4+2]-Annulations of Azoalkenes Under Mild Conditions

Published on: July 17, 2020

6.7K

Related Experiment Videos

Last Updated: Jan 23, 2026

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
09:44

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

Published on: March 3, 2015

9.9K
Author Spotlight: High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography
06:19

Author Spotlight: High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

Published on: March 10, 2023

5.6K
Cercosporin-Photocatalyzed [4+1]- and [4+2]-Annulations of Azoalkenes Under Mild Conditions
07:12

Cercosporin-Photocatalyzed [4+1]- and [4+2]-Annulations of Azoalkenes Under Mild Conditions

Published on: July 17, 2020

6.7K

Area of Science:

  • Biotechnology
  • Microbial Chemistry
  • Enzyme Engineering

Background:

  • R-2-(4-hydroxyphenoxy)propionic acid (R-HPPA) is a crucial intermediate for enantiomerically pure phenoxypropionic acid herbicides.
  • Microbial hydroxylation of R-2-phenoxypropionic acid (R-PPA) is a key step in R-HPPA biosynthesis, requiring specific hydroxylases.

Purpose of the Study:

  • To develop an efficient high-throughput screening method for identifying microorganisms with enhanced R-HPPA biosynthesis capabilities.
  • To optimize the screening assay for rapid evaluation of microbial R-HPPA production.

Main Methods:

  • A colorimetric assay was developed based on the oxidation of R-HPPA to a colored quinone-type compound using potassium dichromate.
  • Absorbance at 570 nm was used to quantify R-HPPA concentration and evaluate biosynthetic capacity.
  • The method was applied to screen Beauveria bassiana mutants for improved R-HPPA production.

Main Results:

  • An optimized high-throughput screening method was successfully established.
  • A Beauveria bassiana mutant (C-7) with significantly enhanced R-HPPA biosynthesis was identified from 1920 screened mutants.
  • Mutant C-7 exhibited 12.5-fold higher biomass and 38.19-fold higher R-HPPA titer compared to the original strain at 20 g/L R-PPA.

Conclusions:

  • The developed high-throughput screening method is effective for rapidly identifying microbial strains with enhanced R-HPPA biosynthesis.
  • This method facilitates the selection of high-performing mutants, such as Beauveria bassiana C-7, for industrial applications.
  • The screening approach holds potential for discovering strains producing other valuable phenolic compounds.