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Identification of Macrophage Genotype and Key Biological Pathways in Circulating Angiogenic Cell Transcriptome.
Bert R Everaert1,2, Steven J Van Laere3, Robrecht Lembrechts1
1Laboratory of Cell Biology and Histology, University of Antwerp, Antwerp, Belgium.
Stem Cells International
|June 14, 2019
Summary
Circulating angiogenic cells (CAC) are genetically similar to regulatory M2c macrophages, not endothelial cells. This finding clarifies the genotype and immune-modulatory function of CAC in vascular biology.
Area of Science:
- Cell Biology
- Immunology
- Vascular Biology
Background:
- Circulating angiogenic cells (CAC) play a role in vascular biology.
- The precise cell type and function of CAC remain debated.
Purpose of the Study:
- To investigate the genotype and function of in vitro cultured CAC (CACiv).
- To compare CACiv with other cell types, including monocytic cells and macrophages.
Main Methods:
- Analysis of publicly available gene expression data sets.
- Transcriptome analysis of CACiv.
- Quantitative PCR (qPCR) to compare gene expression between CACiv and CD14+ monocytic cells.
Main Results:
- CACiv transcriptome closely resembles tissue macrophages, particularly M2c macrophages.
- CACiv exhibit an immune-modulatory cytokine profile similar to tumor-associated macrophages (TAM).
- Identified novel pathways in CACiv, including riboflavin metabolism and LXR/RXR, FXR/RXR signaling.
- No evidence of endothelial transdifferentiation was found in CACiv.
Conclusions:
- CACiv are genotypically identified as regulatory M2c macrophages.
- CACiv do not show characteristics of endothelial differentiation.
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