Related Experiment Video

Updated: Jan 23, 2026

Author Spotlight: Visualizing Single-Stranded DNA During DNA Repair for Therapeutic Insights
08:30

Author Spotlight: Visualizing Single-Stranded DNA During DNA Repair for Therapeutic Insights

Published on: December 22, 2023

3.3K

NFE2L3 Inhibition Induces Cell Cycle Arrest at the G0/G1 Phase in Colorectal Cancer Cells through Downregulating

Lihua Zhang1,2, Dong-Li Hu1,2, Baiyou Tang1,2

  • 1Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, China.

Disease Markers
|June 14, 2019
PubMed

Insights

Nuclear factor, erythroid 2-like 3 (NFE2L3) acts as a potential biomarker for colorectal cancer. Its downregulation induces G0/G1 cell cycle arrest by affecting CCND1 and pRb1-ser807/811 expression.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • The molecular mechanisms driving colorectal cancer (CRC) development are not fully understood.
  • Identifying reliable biomarkers for CRC is crucial for early detection and targeted therapy.

Purpose of the Study:

  • To identify novel gene expression biomarkers associated with colorectal cancer development.
  • To investigate the functional role of the nuclear factor, erythroid 2-like 3 (NFE2L3) gene in colorectal cancer progression.

Main Methods:

  • Gene expression profiling analysis of 380 CRC patients and 51 healthy controls using R software.
  • Real-time PCR and flow cytometry to validate NFE2L3 expression and its effect on cell cycle progression.
  • Western blot analysis to assess the impact of NFE2L3 inhibition on key cell cycle regulatory proteins (CCND1, pRb1-ser807/811).

Main Results:

  • 1579 upregulated and 3218 downregulated differentially expressed genes (DEGs) were identified in CRC.
  • NFE2L3 was identified as a significantly upregulated DEG, with higher expression in tumor tissues compared to paratumor tissues.
  • Inhibition of NFE2L3 led to G0/G1 cell cycle arrest and downregulation of CCND1 and pRb1-ser807/811 in CRC cell lines.

Conclusions:

  • NFE2L3 is a potential biomarker for colorectal cancer.
  • Downregulation of NFE2L3 induces G0/G1 cell cycle arrest, likely through the modulation of CCND1 and pRb1-ser807/811.
  • Further research into NFE2L3's role could offer new therapeutic strategies for colorectal cancer.

More Related Videos

Use of the Pyrimidine Analog, 5-Iodo-2′-Deoxyuridine IdU with Cell Cycle Markers to Establish Cell Cycle Phases in a Mass Cytometry Platform08:37

Use of the Pyrimidine Analog, 5-Iodo-2′-Deoxyuridine IdU with Cell Cycle Markers to Establish Cell Cycle Phases in a Mass Cytometry Platform

Published on: October 22, 2021

3.4K
Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes05:22

Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes

Published on: April 13, 2018

11.0K

Related Concept Videos

What is the Cell Cycle?01:04

What is the Cell Cycle?

The cell cycle refers to the sequence of events occurring throughout a typical cell’s life. In eukaryotic cells, the somatic cell cycle has two stages: interphase and the mitotic phase. During interphase, the cell grows, performs its basic metabolic functions, copies its DNA, and prepares for mitotic cell division. Then, during mitosis and cytokinesis, the cell divides its nuclear and cytoplasmic materials, respectively. This generates two daughter cells that are identical to the original...
242.7K
What is the Cell Cycle?00:56

What is the Cell Cycle?

The cell cycle refers to the sequence of events occurring throughout a typical cell’s life. In eukaryotic cells, the somatic cell cycle has two stages: the interphase and the mitotic phase. During interphase, the cell grows, performs its basic metabolic functions, copies its DNA, and prepares for mitotic cell division. Then, during mitosis and cytokinesis, the cell divides its nuclear and cytoplasmic materials, respectively. This generates two daughter cells that are identical to the...
10.1K
The Cell Cycle Control System01:28

The Cell Cycle Control System

The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
5.4K
The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
14.0K
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
7.7K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.8K