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Diverse Papillomavirus Types Induce Endosomal Tubulation
Abida Siddiqa1,2, Paola Massimi1, David Pim1
1Tumour Virology Laboratory, International Centre for Genetic Engineering and Biotechnology, Trieste, Italy.
Frontiers in Cellular and Infection Microbiology
|June 14, 2019
Summary
Vesicle-associated membrane protein A (VAP) is crucial for papillomavirus entry. This study shows VAP and EGFR-dependent endosomal tubulation are conserved mechanisms across diverse papillomavirus types, essential for infection.
Area of Science:
- Virology
- Cell Biology
- Molecular Biology
Background:
- Human papillomavirus type 16 (HPV-16) requires the ER-anchored protein VAP for infectious entry.
- Entry involves endosomal tubulation and transport to the trans-Golgi network (TGN).
Purpose of the Study:
- To investigate if VAP and endosomal tubulation are conserved entry mechanisms across multiple papillomavirus (PV) types.
- To analyze the role of VAP and endosomal tubulation in PV infection from different genera.
Main Methods:
- Infection assays using Pseudovirions (PsVs) from alpha, beta, delta, kappa, and pi papillomavirus genera.
- Analysis of VAP's role and induction of endosomal tubulation during PV infection.
Main Results:
- VAP was found to be essential for infection across all analyzed PV types.
- All tested PV types induced VAP and EGFR-dependent endosomal tubulation.
Conclusions:
- VAP is universally required for papillomavirus infectious entry.
- VAP-induced endocytic tubulation is an evolutionarily conserved mechanism for papillomavirus entry.
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