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Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
Published on: January 29, 2014
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Invited Article: Digital refocusing in quantitative phase imaging for flowing red blood cells
Han Sang Park1, Silvia Ceballos1, Will J Eldridge1
1Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA.
APL Photonics
|June 14, 2019
Summary
Quantitative phase imaging (QPI) can now analyze flowing cells by digitally refocusing images. This advancement is crucial for accurate analysis of cells in motion, improving biomedical diagnostics.
Area of Science:
- Biomedical optics
- Cellular imaging
- Quantitative phase imaging
Background:
- Quantitative phase imaging (QPI) provides high sensitivity for nanoscale cellular features but is limited to static cells.
- Higher throughput methods are needed for QPI to be used in biomedical diagnostics.
- Imaging cells in flow using QPI is under development, requiring accurate quantitative analysis.
Purpose of the Study:
- To develop and analyze methods for accurate quantitative analysis of cells in flow using QPI.
- To address the challenge of focal plane shifts in flowing cells.
- To investigate the influence of optical absorption on refocusing algorithms.
Main Methods:
- Analysis of QPI images of moving red blood cells.
- Development and application of digital refocusing techniques for holographic images.
- Evaluation of different refocusing criteria, considering optical absorption effects.
Main Results:
- Demonstration of digital refocusing for holographic images of flowing red blood cells.
- Analysis of various refocusing approaches and their effectiveness.
- Identification of the impact of optical absorption on refocusing accuracy.
Conclusions:
- Digital refocusing is a viable technique for analyzing QPI images of cells in flow.
- Careful selection of quantitative criteria is essential for accurate refocusing, especially with optical absorption.
- This work advances the potential of QPI for high-throughput biomedical applications.
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