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Histamine uptake by human platelets.

D S Gill1, M A Barradas, D P Mikhailidis

  • 1Department of Chemical Pathology and Human Metabolism, Royal Free Hospital, London, UK.

Clinica Chimica Acta; International Journal of Clinical Chemistry
|September 30, 1987
PubMed
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Human platelet uptake of histamine was investigated. Stirring and sub-aggregatory concentrations of agonists significantly increased histamine uptake, suggesting its use as an index of platelet behavior in certain conditions.

Area of Science:

  • Hematology
  • Pharmacology
  • Biochemistry

Background:

  • Platelets play a crucial role in hemostasis and thrombosis.
  • Histamine is a biogenic amine with various physiological functions.
  • Altered platelet aggregation is observed in conditions like peripheral vascular disease and diabetes mellitus.

Purpose of the Study:

  • To investigate the uptake of histamine by human platelets.
  • To determine the influence of stirring and platelet agonists on histamine uptake.
  • To explore the potential of platelet histamine uptake as an indicator of platelet behavior.

Main Methods:

  • Incubations were performed using platelet-rich plasma with sodium citrate anticoagulant.
  • Histamine uptake was measured at a concentration of 2.5 nmol/L at 37°C.

Related Experiment Videos

  • Platelet aggregometry was used to assess the effect of stirring and agonists (e.g., adrenaline) on histamine uptake.

    • Main Results:

    • Stirring significantly enhanced histamine uptake by human platelets.
    • Sub-aggregatory concentrations of conventional platelet aggregating agents also significantly increased histamine uptake.
    • These findings indicate a modulation of histamine uptake by platelet activation.

    Conclusions:

    • Platelet histamine uptake is influenced by physical (stirring) and chemical (agonists) stimuli.
    • Histamine uptake by platelets may serve as a valuable index for assessing platelet behavior.
    • This index could be particularly useful in conditions with altered platelet aggregation, such as peripheral vascular disease and diabetes mellitus.