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Development of an enzyme-linked immunosorbent assay using recombinant protein antigen for the diagnosis of

Flávia Fonseca Bagno1,2, Lara Carvalho Godoi2,3, Natalia Salazar2

  • 1Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.

Data in Brief
|June 14, 2019
PubMed

We describe here the development of an in-house enzyme linked immunosorbent assay (ELISA) for the diagnostic of Chikungunya virus (CHIKV) infections using a recombinant protein from CHIKV. The recombinant protein gene was designed based on 154 sequences and we used computational methods to predict its structure and antigenic potential. To confirm predictions, the gene coding for the recombinant CHIKV protein (rCHIKVp) was synthetized and expressed in prokaryotic system. Subsequently, the protein was purified by affinity chromatography and used as antigen in an indirect ELISA. We present data regarding the optimization of the recombinant antigen production and preparation of the ELISA to detect IgG against CHIKV in human sera.

Keywords:
Chikungunya virusDiagnosticsE2 Envelope proteinELISARecombinant protein

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