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Updated: Jan 23, 2026

Measurement of In Vitro Integration Activity of HIV-1 Preintegration Complexes
Published on: February 22, 2017
Integrative modeling of the HIV-1 ribonucleoprotein complex
David S Goodsell1,2, Andrew Jewett1, Arthur J Olson1
1Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, United States of America.
Abstract:
A coarse-grain computational method integrates biophysical and structural data to generate models of HIV-1 genomic RNA, nucleocapsid and integrase condensed into a mature ribonucleoprotein complex. Several hypotheses for the initial structure of the genomic RNA and oligomeric state of integrase are tested. In these models, integrase interaction captures features of the relative distribution of gRNA in the immature virion and increases the size of the RNP globule, and exclusion of nucleocapsid from regions with RNA secondary structure drives an asymmetric placement of the dimerized 5'UTR at the surface of the RNP globule.
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