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Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR
Published on: November 4, 2016
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Identification and Differentiation of Coagulase-Negative Staphylococcus aureus by Polymerase Chain Reaction
K R Matthews1, J Roberson2, B E Gillespie2
1Department of Food Science, Rutgers University, New Brunswick, New Jersey 08903.
Journal of Food Protection
|June 15, 2019
Summary
This study introduces a rapid and accurate polymerase chain reaction DNA fingerprinting method to distinguish coagulase-positive Staphylococcus aureus from coagulase-negative strains, improving diagnostic reliability.
Area of Science:
- Microbiology
- Molecular Biology
- Infectious Diseases
Background:
- Staphylococcus aureus is a common cause of infections in humans and animals.
- Most S. aureus strains produce coagulase, but atypical coagulase-negative strains exist.
- Accurate identification of S. aureus strains is crucial for effective treatment and control.
Purpose of the Study:
- To develop and evaluate a polymerase chain reaction (PCR)-based DNA fingerprinting method.
- To differentiate coagulase-positive Staphylococcus aureus (CPSA) from coagulase-negative Staphylococcus aureus (CNSA).
Main Methods:
- Utilized PCR-based DNA fingerprinting technique.
- Evaluated 50 CPSA and 29 CNSA isolates.
- Assessed differentiation based on visible observation and densitometric analysis.
Main Results:
- PCR-based DNA fingerprinting successfully differentiated CNSA from CPSA.
- The method provided clear distinctions through visual and densitometric evaluation.
- Demonstrated rapidity and accuracy compared to conventional methods.
Conclusions:
- PCR-based DNA fingerprinting is a reliable method for distinguishing CPSA from CNSA.
- This technique offers a rapid and accurate alternative to traditional identification methods.
- Improved diagnostic accuracy can lead to better treatment and control strategies for S. aureus infections.
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