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A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury
Published on: June 17, 2020
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Mesenchymal stromal cells modulate the molecular pattern of healing process in tissue-engineered urinary bladder: the
Marta Pokrywczynska1, Marta Rasmus2, Arkadiusz Jundzill2
1Department of Regenerative Medicine, Cell and Tissue Bank, Chair of Urology, Nicolaus Copernicus University in Torun, Ludwik Rydygier Medical College in Bydgoszcz, 85-094, Marii Sklodowskiej Curie 9 Street, 85-094, Bydgoszcz, Poland. marta.pokrywczynska@interia.pl.
Stem Cell Research & Therapy
|June 15, 2019
Summary
Adipose tissue-derived mesenchymal stromal cells (ASCs) significantly alter the molecular healing patterns in tissue-engineered urinary bladders. The Hedgehog signaling pathway is notably upregulated by ASCs during regeneration, offering potential therapeutic targets.
Area of Science:
- Regenerative Medicine
- Biomaterials Science
- Stem Cell Biology
Background:
- The molecular mechanisms of stem cell-induced regeneration in tissue-engineered urinary bladders remain unclear.
- This study investigates the pathways involved in urinary bladder regeneration using adipose tissue-derived mesenchymal stromal cells (ASCs).
Purpose of the Study:
- To explore the molecular pathways associated with the regenerative process in tissue-engineered urinary bladders.
- To identify signaling pathways modulated by ASCs during bladder reconstruction and healing.
Main Methods:
- Rat urinary bladders were reconstructed using bladder acellular matrix (BAM) with or without ASCs.
- Healing was assessed macroscopically, histologically, and molecularly at multiple time points.
- Gene expression analysis utilized microarrays and real-time PCR to identify differentially expressed genes (DEGs).
Main Results:
- Numerous DEGs were identified between ASC-treated and control bladders.
- Pathway analysis revealed modulation of Hedgehog, TGF-β, Jak-STAT, PI3-Akt, and Hippo pathways by ASCs.
- Real-time PCR confirmed upregulation of key genes in the Hedgehog signaling pathway (Shh, Gli1, Smo, Wnt family, Bmp family).
Conclusions:
- ASCs demonstrably alter the molecular profile of healing in tissue-engineered urinary bladders.
- Identified signaling pathways, particularly Hedgehog, represent potential targets for future regenerative therapies.
- This research provides evidence for ASCs' role in promoting urinary bladder regeneration through specific molecular signaling.
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