Improved Performance of Electrochemically Synthesized Polypyrrole Nanofiber Array-Based Amperometric Glucose
Pramila Jakhar1, Mayoorika Shukla1, Vipul Singh1
1Molecular and Nanoelectronics Research Group (MNRG), Discipline of Electrical Engineering, Indian Institute of Technology Indore, Indore 453552, Madhya Pradesh, India.
Journal of Nanoscience and Nanotechnology
|June 15, 2019
Summary
This study presents a novel, highly sensitive glucose biosensor using crosslinked enzymes on polypyrrole nanofibers. This template-free electrochemical approach significantly enhances sensitivity and response time for glucose detection.
Area of Science:
- Electrochemistry
- Materials Science
- Biosensors
Background:
- Electrochemical biosensors are crucial for detecting biomolecules like glucose.
- Enzyme immobilization techniques significantly impact biosensor performance.
- Developing sensitive and rapid glucose biosensors remains an active research area.
Purpose of the Study:
- To develop a novel, high-sensitivity, template-free glucose biosensor.
- To investigate the effect of enzyme immobilization methods on biosensor performance.
- To optimize glucose detection using polypyrrole nanofiber arrays.
Main Methods:
- Electropolymerization of polypyrrole nanofiber array (PNA) on a Pt-coated glass substrate.
- Enzyme (glucose oxidase, GOx) immobilization via physical adsorption and crosslinking.
- Amperometric transduction for biosensor analysis.
- Comparative performance evaluation of different immobilization strategies.
Main Results:
- Crosslinked GOx immobilization significantly improved glucose biosensor sensitivity (six-fold increase) and response time (~30 s).
- The developed biosensor achieved a high sensitivity of 25.9 mA-cm-2-M-1 and a linear range of 0.1-2.6 mM.
- Demonstrated the substantial impact of enzyme immobilization technique on electrochemical enzymatic glucose biosensor performance.
Conclusions:
- Crosslinking is a superior enzyme immobilization method for enhancing glucose biosensor performance.
- The template-free electrochemical synthesis approach offers a promising route for developing advanced biosensors.
- This novel glucose biosensor exhibits high sensitivity and rapid response, suitable for various applications.
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