Improved Performance of Electrochemically Synthesized Polypyrrole Nanofiber Array-Based Amperometric Glucose Biosensor via Crosslinking Technique

Herein, we report a novel high sensitivity enzymatic template free glucose biosensor in which enzymes have been immobilized via crosslinking over electropolymerized Polypyrrole nanofiber array (PNA). The nanostructured PPy film was electropolymerized over a Pt-coated glass substrate by the potentiostatic deposition method. Further, the enzyme (GOx) was immobilized over the as-prepared PNA electrode utilizing physical adsorption and crosslinking immobilization method, and the fabricated biosensors were analyzed using amperometric transduction method. The performance of both the types of the biosensor was compared, and further investigations demonstrated the improvement in glucose biosensor response for the electrode utilizing crosslinked GOx. The response time of the prepared biosensor was significantly improved for crosslinked GOx along with nearly six times augmentation in the sensitivity for the same. Among two types of biosensors, the crosslinked electrode based biosensor has shown a high sensitivity of 25.9 mA-cm^-2-M^-1 and an improved response time of ~30 s with a reasonable linear range of 0.1-2.6 mM which has been reported for the first time via template free electrochemical synthesis approach. The observed results have shown the significant effect of the enzyme immobilization technique on the performance of electrochemical enzymatic glucose biosensor.