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Updated: Jul 14, 2026
09:42
Generation of Murine Monoclonal Antibodies by Hybridoma Technology
Published on: January 2, 2017
A human hybrid hybridoma
R F Tiebout1, F van Boxtel-Oosterhof, E A Stricker
1Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Journal of Immunology (Baltimore, Md. : 1950)
|November 15, 1987
Summary
Researchers successfully created human hybrid hybridomas, enabling the production of bispecific antibodies. This breakthrough combines cell transformation and xenohybridization techniques for novel antibody development.
Area of Science:
- Immunology
- Cell Biology
- Biotechnology
Background:
- Hybrid hybridomas are cell lines created by fusing two antibody-producing cells.
- Previous research focused on murine hybrid hybridomas for bispecific antibody production.
- The feasibility of constructing human hybrid hybridomas remained unexplored.
Purpose of the Study:
- To investigate the feasibility of creating human hybrid hybridomas.
- To produce bispecific monoclonal antibodies using human cell lines.
Main Methods:
- Fusion of an Epstein-Barr virus-transformed human cell line producing anti-tetanus toxoid IgG1 kappa antibodies with a human-mouse xenohybrid cell line producing anti-hepatitis B surface antigen IgG1 lambda antibodies.
- Selection of hybrid hybridoma cells using a culture medium containing azaserine/hypoxanthine and ouabain.
- Analysis of hybrid antibody nature using antigen-specific immunoassays.
Main Results:
- Successful construction of human hybrid hybridomas.
- Demonstration that these hybrid hybridomas produce bispecific antibodies.
- Validation of combined transformation and xenohybridization techniques.
Conclusions:
- Human hybrid hybridomas can be constructed using combined transformation and xenohybridization.
- This method enables the production of human bispecific antibodies.
- Potential for developing novel therapeutic antibodies.
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