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Related Experiment Video

Updated: Jul 14, 2026

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Published on: January 2, 2017

A human hybrid hybridoma.

R F Tiebout¹, F van Boxtel-Oosterhof, E A Stricker

¹Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam.

Journal of Immunology (Baltimore, Md. : 1950)

|November 15, 1987

Summary

Researchers successfully created human hybrid hybridomas, enabling the production of bispecific antibodies. This breakthrough combines cell transformation and xenohybridization techniques for novel antibody development.

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Area of Science:

Immunology
Cell Biology
Biotechnology

Background:

Hybrid hybridomas are cell lines created by fusing two antibody-producing cells.
Previous research focused on murine hybrid hybridomas for bispecific antibody production.
The feasibility of constructing human hybrid hybridomas remained unexplored.

Purpose of the Study:

To investigate the feasibility of creating human hybrid hybridomas.
To produce bispecific monoclonal antibodies using human cell lines.

Main Methods:

Fusion of an Epstein-Barr virus-transformed human cell line producing anti-tetanus toxoid IgG1 kappa antibodies with a human-mouse xenohybrid cell line producing anti-hepatitis B surface antigen IgG1 lambda antibodies.
Selection of hybrid hybridoma cells using a culture medium containing azaserine/hypoxanthine and ouabain.
Analysis of hybrid antibody nature using antigen-specific immunoassays.

Main Results:

Successful construction of human hybrid hybridomas.
Demonstration that these hybrid hybridomas produce bispecific antibodies.
Validation of combined transformation and xenohybridization techniques.

Conclusions:

Human hybrid hybridomas can be constructed using combined transformation and xenohybridization.
This method enables the production of human bispecific antibodies.
Potential for developing novel therapeutic antibodies.