Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Data Reporting and Recording01:24

Data Reporting and Recording

5.4K
Reporting and recording are crucial in data documentation. The timely, thorough, and accurate documentation of facts is essential when recording patient data. Failure to record findings during an assessment or interpretation of a problem will result in loss of information and make the patient document unreliable. The reader is left with general impressions if the information is not specific. A recording is documenting data of the individual's health information in a traceable, secure, and...
5.4K
Types of Reports I: Hands-off Report01:25

Types of Reports I: Hands-off Report

1.5K
A hand-off report, also known as a change-of-shift report, is a crucial nursing process that ensures the smooth transition of patient care responsibilities between nursing staff.
Following are the key components and categories of hand-off reports:
Purpose and Process:
1.5K
Types of Reports II: Incident or Occurrence Report01:21

Types of Reports II: Incident or Occurrence Report

1.2K
An Incident or Occurrence Report in a healthcare setting is a crucial document used to record any unexpected occurrence that may or may not have affected a patient, employee, or visitor. Such reports are critical to improving patient safety and include all details leading up to and including the event.
Purposes:
In the healthcare industry, reports play a crucial role in documenting incidents within an agency. The primary objective of these reports is to ensure patient safety, uphold the...
1.2K
Healthcare Associated Infections I: Iatrogenic, Exogenic and Endogenic01:26

Healthcare Associated Infections I: Iatrogenic, Exogenic and Endogenic

5.4K
Healthcare-associated infections (HAIs) occur in a healthcare facility while a person receives care for another ailment. This category also includes work-related infections among healthcare staff.
HAIs significantly increase the cost of health care. Extended stays in healthcare institutions, increased disability, increased costs of medications, including specialized antibiotics, and prolonged recovery times add to the patient's expenses and the healthcare institution and funding bodies.
5.4K
Types of Reports III: Telephone and Verbal Reports01:26

Types of Reports III: Telephone and Verbal Reports

995
Telephone and Verbal Reports in healthcare settings are two communication methods for conveying therapeutic instructions from healthcare providers to nurses or other healthcare staff.
Here's an overview of each type:
Telephone Orders
995
Reporter Genes02:11

Reporter Genes

13.1K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
13.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phase-based optoretinographic measurements of cones with a raster-scanning adaptive optics OCT are highly repeatable.

Biomedical optics express·2026
Same author

Roles of sonic hedgehog signaling in retinal patterning and neurogenesis during mammalian eye development.

Development (Cambridge, England)·2026
Same author

Dark adaptation of cone photoreceptor responses is revealed by optoretinography.

bioRxiv : the preprint server for biology·2026
Same author

Uncoupling neocortical neuron fate and migration via a Let-7-RBX2 axis.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Whole retina light-evoked optoretinography (ORG) under different retina hydration levels: modeling of Bruch's membrane ORGs.

Biomedical optics express·2026
Same author

Oriented cell divisions induce basal progenitors and regulate neural expansion across tissues and species.

Science advances·2026

Related Experiment Video

Updated: Jan 23, 2026

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

7.5K

A Novel Reporter Mouse Uncovers Endogenous Brn3b Expression.

Adam M Miltner1, Yesica Mercado-Ayon2, Simranjeet K Cheema3

  • 1Department of Cell Biology and Human Anatomy, University of California-Davis, Davis, CA 95616, USA. ammiltner@ucdavis.edu.

International Journal of Molecular Sciences
|June 15, 2019
PubMed
Summary
This summary is machine-generated.

We developed a Brn3b-mCherry reporter mouse using CRISPR technology. This tool allows noninvasive live imaging of retinal ganglion cells (RGCs) for monitoring visual system development and disease.

Keywords:
CRISPR-Cas9optic stalkretinal developmentretinal ganglion cells (RGCs)retinal imagingscanning laser ophthalmoscopy (SLO)

More Related Videos

Visualization of IL-22-expressing Lymphocytes Using Reporter Mice
10:30

Visualization of IL-22-expressing Lymphocytes Using Reporter Mice

Published on: January 25, 2017

9.3K
In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters
07:44

In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters

Published on: July 4, 2018

8.8K

Related Experiment Videos

Last Updated: Jan 23, 2026

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

7.5K
Visualization of IL-22-expressing Lymphocytes Using Reporter Mice
10:30

Visualization of IL-22-expressing Lymphocytes Using Reporter Mice

Published on: January 25, 2017

9.3K
In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters
07:44

In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters

Published on: July 4, 2018

8.8K

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Genetics

Background:

  • Brn3b (Pou4f2) is a crucial transcription factor for neuronal development in the central nervous system.
  • Retinal ganglion cells (RGCs) are vital for transmitting visual information from the retina to the brain.

Purpose of the Study:

  • To create a Brn3b-mCherry reporter mouse line to visualize Brn3b expression.
  • To establish a noninvasive method for longitudinal monitoring of RGCs.

Main Methods:

  • CRISPR-based genetic engineering was employed to generate the Brn3b-mCherry reporter mouse.
  • Expression patterns of Brn3b-mCherry were analyzed in various neural tissues.
  • Scanning Laser Ophthalmoscopy (SLO) was used for live imaging of RGCs.

Main Results:

  • The Brn3b-mCherry reporter accurately reflects endogenous Brn3b expression in the retina, optic tracts, midbrain tectum, and trigeminal ganglia.
  • Novel Brn3b expression was detected in early optic stalk neuroectodermal cells.
  • High fluorescent intensity enabled noninvasive live imaging of RGCs via SLO.

Conclusions:

  • The Brn3b-mCherry reporter mouse provides a faithful readout of Brn3b expression during development.
  • This reporter line offers a novel, noninvasive tool for in vivo, longitudinal studies of RGCs and visual system development.