Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Combinatorial Gene Control02:33

Combinatorial Gene Control

9.5K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
9.5K
Gene Flow02:39

Gene Flow

37.6K
Gene flow is the transfer of genes among populations, resulting from either the dispersal of gametes or from the migration of individuals.
37.6K
Gene Families01:57

Gene Families

9.9K
Gene families consist of groups of genes proposed to have originated from a common ancestor. Typically these arise through events in which a gene or genes are mistakenly duplicated during cell division. Unlike their parent genes (which are subject to selection pressure to maintain function), these gene copies do not need to preserve their sequences and may evolve at a relatively faster rate.
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
9.9K
Gene Therapy00:59

Gene Therapy

27.4K
Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...
27.4K
What is Gene Expression?01:42

What is Gene Expression?

195.1K
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
195.1K
Gene Conversion02:08

Gene Conversion

10.6K
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
10.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Complete genome sequence of multidrug-resistant <i>Edwardsiella ictaluri</i> A21-00102 isolated from channel catfish (<i>Ictalurus punctatus</i>) in the United States.

Microbiology resource announcements·2026
Same author

Hi-C Metagenome Deconvolution of Double-Crested Cormorant (<i>Nannopterum auritum</i>) Fecal Samples Demonstrates Feasibility of Linking Microbial Genomes, AMR Genes, and Mobile Elements in Avian Microbiomes.

Microorganisms·2026
Same author

Recombinant TadZ from the type IVc pilus system induces protective immunity against virulent <i>Aeromonas hydrophila</i> in channel catfish (<i>Ictalurus punctatus</i>).

Frontiers in immunology·2026
Same author

Histopathologic scoring system for low-salinity water (freshwater) exposure lesions in bottlenose dolphins (<i>Tursiops truncatus</i>).

Veterinary pathology·2026
Same author

<i>Plesiomonas shigelloides</i> as an Emerging Pathogen in Catfish Aquaculture: A Case from a South Texas Commercial Farm.

Microorganisms·2026
Same author

Investigation mechanisms of action and resistance of Edwardsiella ictaluri to trans-cinnamaldehyde.

PloS one·2026

Related Experiment Video

Updated: Jan 23, 2026

Listeria monocytogenes Infection of the Brain
05:02

Listeria monocytogenes Infection of the Brain

Published on: October 2, 2018

13.2K

Efficient Gene Deletion Method for Listeria monocytogenes.

Hossam Abdelhamed1, Attila Karsi1, Mark L Lawrence2

  • 1College of Veterinary Medicine, Mississippi State University, Mississippi State, MS, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 15, 2019
PubMed
Summary

Researchers developed a new suicide plasmid, pHoss1, for efficient markerless gene deletion in Listeria monocytogenes. This tool aids in understanding gene function by enabling precise genetic modifications in various bacterial strains.

Keywords:
Gene deletionListeria monocytogenesPxyl/tetO promoterpHoss1secY

More Related Videos

Oral Transmission of Listeria monocytogenes in Mice via Ingestion of Contaminated Food
08:38

Oral Transmission of Listeria monocytogenes in Mice via Ingestion of Contaminated Food

Published on: May 6, 2013

20.0K
Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-&#947; Responses
10:10

Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses

Published on: November 16, 2016

14.2K

Related Experiment Videos

Last Updated: Jan 23, 2026

Listeria monocytogenes Infection of the Brain
05:02

Listeria monocytogenes Infection of the Brain

Published on: October 2, 2018

13.2K
Oral Transmission of Listeria monocytogenes in Mice via Ingestion of Contaminated Food
08:38

Oral Transmission of Listeria monocytogenes in Mice via Ingestion of Contaminated Food

Published on: May 6, 2013

20.0K
Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-&#947; Responses
10:10

Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses

Published on: November 16, 2016

14.2K

Area of Science:

  • Molecular Biology
  • Microbiology
  • Bacterial Genetics

Background:

  • Gene function is elucidated through inactivation or deletion.
  • Markerless gene deletion is crucial for precise genetic manipulation in bacteria.
  • Listeria monocytogenes is a significant bacterial pathogen requiring genetic study.

Purpose of the Study:

  • To develop a novel suicide plasmid for markerless gene deletion in Listeria monocytogenes.
  • To provide a tool for efficient and versatile genetic modification in L. monocytogenes strains.
  • To facilitate the investigation of gene function through targeted gene deletion.

Main Methods:

  • Development of the pHoss1 suicide plasmid, incorporating pMAD backbone, secY antisense cassette, inducible promoter (Pxyl/tetO), heat-sensitive origin of replication, restriction sites, and erythromycin resistance.
  • Application of pHoss1 for introducing markerless gene deletions in various Listeria monocytogenes strains.
  • Methodology description for utilizing pHoss1 in L. monocytogenes gene deletion experiments.

Main Results:

  • The pHoss1 plasmid was successfully constructed and characterized.
  • pHoss1 demonstrated high efficiency in facilitating markerless gene deletions across different L. monocytogenes strains.
  • The developed method provides a reliable approach for genetic engineering in Listeria.

Conclusions:

  • pHoss1 is an effective tool for markerless gene deletion in Listeria monocytogenes.
  • This plasmid facilitates efficient genetic manipulation, aiding in the study of gene function.
  • The described method offers a valuable resource for researchers working with L. monocytogenes.