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Related Experiment Video

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In Vitro Dynamic Phenotyping for Testing Novel Mobilizing Agents.

Motomu Tanaka^1,2

¹Physical Chemistry of Biosystems, Institute of Physical Chemistry, Heidelberg University, Heidelberg, Germany. tanaka@uni-heidelberg.de.

Methods in Molecular Biology (Clifton, N.J.)

|June 15, 2019

Summary

A new method quantifies how mobilization agents affect human hematopoietic stem and progenitor cells (HSPC) dynamics. This approach analyzes cell shape and motion changes, offering insights into treatment effects on HSPC behavior.

Keywords:

Cell adhesion Cell migration Supported membrane Theoretical model

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Area of Science:

Biophysics
Cellular Dynamics
Hematopoiesis

Background:

Mobilization agents are crucial for hematopoietic stem and progenitor cell (HSPC) collection.
Existing methods for assessing HSPC dynamics are often indirect or complex.
Understanding HSPC response to extrinsic factors is vital for effective stem cell therapies.

Purpose of the Study:

To introduce a novel method for quantifying the dynamic behavior of human HSPC.
To investigate the influence of mobilization agents on HSPC shape and migration.
To provide a quantitative framework for evaluating therapeutic interventions on HSPC.

Main Methods:

Fabrication of a bone marrow niche model using supported lipid membranes with controlled ligand densities.
Time-lapse live cell imaging of human HSPC (CD34+ cells) using phase-contrast microscopy or microinterferometry.
Analysis of cell shape dynamics (deformation, persistence, energy consumption) and migration trajectories.

Main Results:

The method captures detailed information on HSPC shape dynamics, including deformation modes and energy consumption.
Analysis revealed how mobilization agents alter HSPC migration patterns.
Quantitative indices derived from cell dynamics correlate with the effects of extrinsic factors.

Conclusions:

The developed method offers a powerful tool to study HSPC dynamics in response to various agents.
This approach enables quantitative classification of the influence of mobilization agents, inhibitors, and other treatments.
The findings contribute to a deeper understanding of HSPC behavior in the context of stem cell mobilization and therapy.