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Updated: Jan 23, 2026

Staining and High-Resolution Imaging of Three-Dimensional Organoid and Spheroid Models
Published on: March 27, 2021
Stain-free subcellular-resolution astrocyte imaging using third-harmonic generation
Abstract:
We demonstrate stain-free, high-contrast, subcellular-resolution imaging of astroglial cells using epi-detected third-harmonic generation (THG). The astrocyte-imaging capability of THG is verified by colocalizing THG images with fluorescence images of astrocytes expressing a genetically encodable fluorescent reporter. We show that THG imaging with an optimized point-spread function can reliably detect significant subcellular features of astrocytes, including cell nuclei, as well as the soma shape and boundaries.
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