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Updated: Jan 23, 2026

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
Published on: March 21, 2018
Highly sensitive detection of microcystin-LR under visible light using a self-powered photoelectrochemical aptasensor
Lin Tang1, Xilian Ouyang1, Bo Peng1
1College of Environmental Science and Engineering, Hunan University, Changsha 410082, China. tanglin@hnu.edu.cn zgming@hnu.edu.cn and Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, Hunan, China.
Abstract:
Based on the unique photoelectrochemical properties of a CoO/Au/g-C3N4 Z-scheme heterojunction, a self-powered photoelectrochemical (PEC) aptasensor was constructed for the detection of microcystin-leucine arginine (MC-LR). Z-scheme heterojunctions can promote the separation of a photo-induced electron-hole pair, and the surface plasmonic resonance (SPR) of Au nanoparticles can significantly enhance the adsorption of visible light. Importantly, MC-LR molecules were captured by aptamers initially immobilized on the modified electrode due to their high affinity, and then oxidized by the photogenerated holes, which caused an amplified photocurrent signal, allowing the quantitative analysis of MC-LR by measuring the photocurrent intensity change. This PEC MC-LR aptasensor showed high sensitivity and selectivity within a wide linear response range from 0.1 pM to 10 nM and a detection limit of 0.01 pM. The application of this sensor in the analysis of lake water samples provided accurate results with a relative standard deviation (RSD) of 2.6%-4.2%.
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