Characterisation of the First Bovine Parainfluenza Virus 3 Isolate Detected in Cattle in Turkey
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Harun Albayrak1, Zafer Yazici2, Emre Ozan3
1Department of Virology, Faculty of Veterinary Medicine, Ondokuz Mayis University, 55200 Samsun, Turkey. harunalbayrak55@msn.com.
Abstract:
A respiratory disease outbreak on a cattle farm in northern Turkey produced respiratory tract symptoms and severe pneumonia symptoms in 20 calves. Eight calves died, and a lung specimen from one carcass was analysed for bacteria and for viruses of the Bovine respiratory diseases complex. Bacteriological analysis was negative, but antigen detection ELISA and RT-PCR results indicated the presence of Bovine parainfluenza virus (BPIV). Virus isolation succeeded on Madin-Darby Bovine Kidney cells, and subsequent whole genome sequencing and phylogenetic analysis identified BPIV-3c. This is the first report of BPIV-3c isolation from cattle in Turkey, indicating the need for more virological and epidemiological studies.
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