Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Hybrid Zones

Hybrid Zones

Hybrid zones are narrow regions where two closely related species interact, mate, and produce hybrids. Relative to either parent species, hybrids may possess distinct phenotypic or genetic differences that impact their survival and reproductive success. The genetic variances introduced by hybridization influence species diversity and speciation processes within the hybrid zone.

Hybridization of Atomic Orbitals II

Hybridization of Atomic Orbitals II

sp3d and sp3d 2 Hybridization

Hybridization of Atomic Orbitals I

Hybridization of Atomic Orbitals I

The mathematical expression known as the wave function, ψ, contains information about each orbital and the wavelike properties of electrons in an isolated atom. When atoms are bound together in a molecule, the wave functions combine to produce new mathematical descriptions that have different shapes. This process of combining the wave functions for atomic orbitals is called hybridization and is mathematically accomplished by the linear combination of atomic orbitals. The new orbitals that...

Light Acquisition

Light Acquisition

In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.

Water and Mineral Acquisition

Water and Mineral Acquisition

Specialized tissues in plant roots have evolved to capture water, minerals, and some ions from the soil. Roots exhibit a variety of branching patterns that facilitate this process. The outermost root cells have specialized structures called root hairs that increase the root surface, thus increasing soil contact. Water can passively cross into roots, as the concentration of water in the soil is higher than that of the root tissue. Minerals, in contrast, are actively transported into root cells.

How Data are Classified: Numerical Data

How Data are Classified: Numerical Data

Data that are countable or measurable in specific units are called numerical or quantitative data. Quantitative data are always numbers. Quantitative data are the result of counting or measuring the attributes of a population. Amount of money, pulse rate, weight, number of people living in a town, and number of students who opt for statistics are examples of quantitative data.
Quantitative data may be either discrete or continuous. All quantitative data that take on only specific numerical...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Multistage Tandem Mass Spectrometry Using an Electron-Activated Dissociation Device with a Linear Ion Trap and Structural Identification of Cardiolipins.

Analytical chemistry·2025

Same author

General Screening and Multiple Dissociation Methods for Complementary LC-MS Analysis of Pesticides in Beverages: Potential and Pitfalls.

Analytical chemistry·2025

Same author

Dose, Kidney Function, and a Drug-Excipient Interaction Impair Mycophenolate Mofetil Prodrug Activation in Kidney Transplant Recipients.

European journal of drug metabolism and pharmacokinetics·2025

Same author

Perturbation-Modulated Native Mass Spectrometry Excludes a Nonspecific Drug Target Protein Binder Based on Conformation Stability Change.

Analytical chemistry·2025

Same author

De Novo Structural Elucidation of Acylglycerols by Supercritical Fluid Chromatography and Collision-Induced Dissociation of Electron-Deficient Precursor Ions.

Analytical chemistry·2025

Same author

High-throughput liquid chromatography-vacuum differential mobility spectrometry-mass spectrometry for the analysis of isomeric drugs of abuse in human urine.

Drug testing and analysis·2024

Search research articles

Related Experiment Video

Updated: Jan 23, 2026

Data Acquisition Protocol for Determining Embedded Sensitivity Functions

Data Acquisition Protocol for Determining Embedded Sensitivity Functions

Published on: April 20, 2016

Hybrid SWATH/MS and HR-SRM/MS acquisition for phospholipidomics using QUAL/QUANT data processing.

Michel Raetz¹, Eva Duchoslav², Ron Bonner³

¹Life Sciences Mass Spectrometry, Department of Inorganic and Analytical Chemistry, University of Geneva, 24 Quai Ernest Ansermet, CH-1211, Geneva 4, Switzerland.

Analytical and Bioanalytical Chemistry

|June 16, 2019

Summary

A novel hybrid SWATH/MS and HR-SRM/MS method enables simultaneous lipidomics analysis. This approach quantifies numerous lipid species and sum compositions in human plasma, improving lipid identification and offering broad applicability.

Keywords:

Data processing Glycerophospholipids HILIC Plasma QUAL/QUANT SWATH

More Related Videos

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

Published on: March 19, 2021

Data Acquisition and Analysis In Brainstem Evoked Response Audiometry In Mice

Data Acquisition and Analysis In Brainstem Evoked Response Audiometry In Mice

Published on: May 10, 2019

Related Experiment Videos

Last Updated: Jan 23, 2026

Data Acquisition Protocol for Determining Embedded Sensitivity Functions

Data Acquisition Protocol for Determining Embedded Sensitivity Functions

Published on: April 20, 2016

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

Published on: March 19, 2021

Data Acquisition and Analysis In Brainstem Evoked Response Audiometry In Mice

Data Acquisition and Analysis In Brainstem Evoked Response Audiometry In Mice

Published on: May 10, 2019

Area of Science:

Analytical Chemistry
Biochemistry
Mass Spectrometry

Background:

Lipidomics research requires sensitive and comprehensive analytical methods.
Current techniques face limitations in simultaneous quantification of diverse lipid species and classes.

Purpose of the Study:

To develop a hybrid SWATH/MS and HR-SRM/MS acquisition strategy for enhanced lipidomics analysis.
To enable simultaneous monitoring of lipid sum compositions and individual lipid species.
To improve lipid identification and quantification in complex biological samples like human plasma.

Main Methods:

Integration of SWATH/MS and HR-SRM/MS with chromatographic lipid class separation.
Utilized multiple unit mass windows and precursor selection windows for data acquisition.
Employed a data processing strategy based on prediction and combinatorial analysis for lipid identification.
Assessed workflow robustness and precision using technical triplicates.

Main Results:

Simultaneous monitoring of lipid sum compositions (MS1) and up to 48 lipids/class (MS2) achieved.
Quantified 240 lipid sum compositions from five phospholipid classes in MS2.
Successfully quantified an average of 92 lipid sum compositions and 75 lipid species in human plasma.
Demonstrated improved lipid identification through predictive data processing.

Conclusions:

The developed hybrid SWATH/MS and HR-SRM/MS approach offers a robust and precise workflow for lipidomics.
This method significantly enhances the scope and accuracy of lipid profiling in human plasma.
The approach is versatile and applicable to various analytes and sample types beyond phospholipids.