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Updated: Jan 23, 2026

An Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons
Published on: July 11, 2020
Dipeptide Mimetic of the BDNF Loop 4 Possesses Analgetic Activity
T A Gudasheva1, M A Konstantinopolsky2, A V Tarasiuk2
1Zakusov Research Institute of Pharmacology, Russian Academy of Medical Sciences, 125315, Moscow, Russia. tata-sosnovka@mail.ru.
Abstract:
Previously, we synthesized a dimeric dipeptide mimetic of the brain-derived neurotrophic factor (BDNF) loop 4, GSB-106, which, similarly to BDNF, activated TrkB, PI3K/AKT, and MAPK/ERK. When administered systemically, it exhibited neuroprotective, antidepressant, and antidiabetic activities and stimulated neurogenesis and synaptogenesis. In this study, we established that GSB-106 also exhibits the analgesic activity, typical for BDNF, which was revealed in rats in hot plate and tail flick tests 0.5-48 h after intraperitoneal injection at doses of 0.1 and 1 mg/kg.
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