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Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings
Published on: August 18, 2023
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The Generation of the Human mAb RabD4 Specific to the Rabies Virus Glycoprotein and Characterization Thereof
E N Ilina1, E V Solopova2, T K Aliev3
1Moscow State University, 119991, Moscow, Russia. Ekaterina.iljina.7@yandex.ru.
Doklady. Biochemistry and Biophysics
|June 16, 2019
Summary
Researchers developed a new human antibody, RabD4, that effectively neutralizes rabies virus. This antibody targets the rabies virus glycoprotein and shows high binding and neutralizing activity.
Area of Science:
- Immunology
- Virology
- Biotechnology
Background:
- Rabies virus poses a significant global health threat.
- Developing effective neutralizing antibodies is crucial for rabies prevention and treatment.
Purpose of the Study:
- To generate a novel human monoclonal antibody (mAb) with potent rabies virus-neutralizing capabilities.
- To characterize the antigen-binding and virus-neutralizing activity of the novel antibody.
Main Methods:
- In vitro stimulation of human peripheral B cells from an immunized donor.
- Production of a human monoclonal antibody (mAb) designated RabD4.
- Antigen-binding assays and the Fluorescent Antibody Virus Neutralization (FAVN) test using CVS-11 rabies virus.
Main Results:
- A novel human neutralizing antibody, RabD4, was successfully generated.
- RabD4 demonstrated high antigen-binding activity against rabies virus glycoprotein.
- The antibody exhibited significant virus-neutralizing activity in the FAVN test.
Conclusions:
- The human mAb RabD4 is a promising candidate for rabies immunotherapy.
- Targeting the rabies virus glycoprotein with human mAbs can yield potent neutralizing agents.
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