Related Experiment Video
Updated: Jan 23, 2026

Analysis of Oxidative Stress in Zebrafish Embryos
Published on: July 7, 2014
Bactericidal Effects of Oxidative Stress Generated by EDTA-Fe and Hydrogen Peroxide
1Graduate Institute of Engineering Science and Technology, National Kaohsiung University of Science and Technology.
Abstract:
Hydrogen peroxide is widely used as a disinfectant by generating oxidative stress. The chelate of ethylenediaminetetraacetic acid (EDTA) and ferrous iron (EDTA-Fe) was used to increase oxidative stress and bactericidal effects. The Escherichia coli (E. coli) was added to a beef extract culture medium and treated with various doses of test reagents, including hydrogen peroxide, EDTA-Fe chelate, and antioxidants (vitamin C and vitamin E) . All reagents were scanned individually or in combination to trace potential interference in optical density (OD) measurements and eliminate reagent-related interference. Medium supplemented with 13.79 mM hydrogen peroxide resulted in a large increase in the mortality of E. coli, and the highest disinfection efficiency for EDTA-Fe was observed at a neutral pH. The death of the cell of E. coli was significantly inhibited by the presence of catalase, but not vitamins C and E, suggesting that hydroxyl radicals were not generated during the EDTA-Fe-hydrogen peroxide reaction.
More Related Videos
09:33Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
Published on: February 7, 2018
08:57Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Published on: February 24, 2018
Related Concept Videos
Effects of EDTA on End-Point Detection Methods
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
Oxidation Numbers
Hydrogen Bonds
Hydrogen Bonds Control the World!
Because hydrogen has very weak electronegativity when it binds with a strongly electronegative atom, such as oxygen or nitrogen, electrons in the bond are unequally shared....
Oxidation-Reduction Reactions
EDTA: Chemistry and Properties
EDTA: Conditional Formation Constant
For the equilibrium reaction of the metal with the...