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[Sensitivity to virulent bacteriophages of Pseudomonas aeruginosa strains of different serogroups]
Abstract:
The data on the sensitivity of P. aeruginosa clinical strains to pyocyaneum, a therapeutic and prophylactic bacteriophage preparation, and to individual groups of phages contained in this preparation are presented. Out of 549 P. aeruginosa strains, 16% have proved to be nonlysing cultures. The proportion of phage-sensitive strains prevailed in serogroups 01, 03, 06, 09, while phage-resistant strains prevailed in serogroups 04, 07, 011, as well as among O-nontyped cultures. The expediency of introducing P. aeruginosa strains of different serotypes into the collection of cultures used for the production of pyocyaneum has been shown.
Insights
This study assessed the sensitivity of clinical Pseudomonas aeruginosa strains to the bacteriophage preparation pyocyaneum. Results show varying sensitivity across different serogroups, highlighting the need for diverse strains in pyocyaneum production.
Area of Science:
- Microbiology
- Bacteriology
- Virology
Background:
- Pseudomonas aeruginosa is an opportunistic pathogen causing various infections.
- Bacteriophage therapy offers a potential alternative to antibiotics for P. aeruginosa infections.
- Pyocyaneum is a therapeutic and prophylactic bacteriophage preparation targeting P. aeruginosa.
Purpose of the Study:
- To evaluate the sensitivity of clinical P. aeruginosa strains to pyocyaneum.
- To determine the sensitivity of these strains to individual phage groups within pyocyaneum.
- To assess the distribution of phage sensitivity across different P. aeruginosa serogroups.
Main Methods:
- Testing the lysis of 549 clinical P. aeruginosa isolates using pyocyaneum.
- Analyzing the sensitivity patterns based on P. aeruginosa serogroups (O1, O3, O4, O6, O7, O9, O11, and non-typed).
Main Results:
- 16% of the tested P. aeruginosa strains were non-lysing cultures.
- Phage-sensitive strains were more common in serogroups O1, O3, O6, and O9.
- Phage-resistant strains predominated in serogroups O4, O7, O11, and among non-typed cultures.
Conclusions:
- Significant variation in phage sensitivity exists among clinical P. aeruginosa strains.
- Serotype distribution impacts P. aeruginosa susceptibility to pyocyaneum.
- Including diverse P. aeruginosa serotypes in pyocyaneum production is recommended for broader efficacy.