Strategies and analytical workflows to extend the dynamic range in quantitative LC-MS/MS analysis
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Updated: Jan 23, 2026

A Quantitative Glycomics and Proteomics Combined Purification Strategy
Published on: March 8, 2016
Emmanuel Njumbe Ediage1, Tania Aerts2, Arnaud Lubin1
1Janssen Research & Development, 2340 Beerse, Belgium.
Abstract:
Aim: To evaluate alternative analytical strategies to extend the dynamic range in quantitative LC-MS/MS. Methods & results: Two approaches based on prior or no prior knowledge of expected exposure levels were evaluated. These approaches make use of two analytical strategies, which include the use of more than one injection volume or dilution of sample extract with solvents or solvent mixtures. A total of 16 compounds with varying logP values were classified into polar and nonpolar groups and used in this evaluation. From the two analytical strategies, three workflows were derived. Conclusion: All three workflows were successfully evaluated and resulted in good accuracy (80-120%) for all the compound groups.
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