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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
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Alternative splicing events during adipogenesis from hMSCs.

Xia Yi1, Yunzhong Yang2, Ping Wu1

  • 1Jiangxi Provincial Key Laboratory of Systems Biomedicine, Jiujiang University, Jiujiang, China.

Journal of Cellular Physiology
|June 18, 2019
PubMed
Summary

This study reveals alternative splicing events during human mesenchymal stem cell (hMSC) adipogenesis, identifying key genes and splicing patterns. These findings offer new insights into fat metabolism regulation and potential therapeutic targets for metabolic disorders.

Keywords:
ACTN1LRP1LTBP4adipogenesis from hMSCsalternative splicing events

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Adipogenesis, the differentiation of stem cells into fat cells, is crucial for fat metabolism but can lead to disorders.
  • Alternative splicing (AS) generates diverse messenger RNA (mRNA) molecules from a single gene, regulating gene expression.
  • The role of AS in adipogenesis from human mesenchymal stem cells (hMSCs) remains incompletely understood.

Purpose of the Study:

  • To globally identify differentially expressed alternative splicing events (ASE) and genes during adipogenesis in hMSCs.
  • To investigate the prevalence and types of AS events occurring throughout the adipogenesis process.
  • To pinpoint specific ASE and genes that may act as key regulators in adipogenesis.

Main Methods:

  • RNA sequencing (RNA-Seq) was employed to capture global gene expression profiles.
  • Bioinformatics analysis was used to identify and quantify alternative splicing events and differentially expressed genes.
  • Data analysis focused on common AS types, including cassette exon, alternative 3' (alt3) and 5' (alt5) splicing.

Main Results:

  • Thousands of ASE were identified across different time points (7, 14, 21, 28 days) of adipogenesis, with cassette exons being the most prevalent.
  • 122 differentially expressed ASE involving 118 genes were identified.
  • Genes like ACTN1, LRP1, and LTBP4 exhibited multiple types of AS events. Notably, alt3 splicing in the transmembrane domain was a significant finding.

Conclusions:

  • This study provides the first comprehensive global transcriptional profiling of ASE during hMSC adipogenesis.
  • Identified ASE and related genes are potential regulators of adipogenesis.
  • These findings offer novel targets for future research into adipogenesis-related metabolic disorders.