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In Ovo Xenografting of Patient-Derived Acute Lymphoblastic Leukemia (ALL) Cells (PDX-ALL)
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MiR-137 inhibits cell proliferation in acute lymphoblastic leukemia by targeting JARID1B

Yiqun Huang1, Yong Zou1, Ruiji Zheng1

  • 1Department of Hematology, Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou, Fujian, China.

European Journal of Haematology
|June 18, 2019
PubMed
Abstract

Insights

MicroRNA-137 (miR-137) is downregulated in acute lymphoblastic leukemia (ALL), and JARID1B directly represses it. Aberrant JARID1B expression may cause ALL through abnormal histone methylation.

Area of Science:

  • Oncology
  • Molecular Biology
  • Epigenetics

Background:

  • Acute lymphoblastic leukemia (ALL) is a hematological malignancy with complex molecular underpinnings.
  • JARID1B is a histone demethylase implicated in various cancers.
  • MicroRNAs (miRNAs) play crucial roles in gene regulation and are often dysregulated in leukemia.

Purpose of the Study:

  • To investigate the functional interaction between JARID1B and miR-137 in ALL.
  • To determine the impact of this interaction on leukemia cell behavior.

Main Methods:

  • Analysis of H3K4me3, H3K4me2, JARID1B, and miR-137 expression in ALL cell lines and patient samples.
  • Gene silencing and overexpression experiments to assess effects on cell proliferation and apoptosis.
  • Dual-luciferase reporter assay to confirm direct interaction between JARID1B and miR-137.

Main Results:

  • Lower levels of H3K4 methylation and miR-137, with elevated JARID1B, were observed in ALL.
  • miR-137 overexpression reduced proliferation and increased apoptosis in ALL cells.
  • JARID1B was identified as a direct target of miR-137, with a negative correlation between their expressions.

Conclusions:

  • miR-137 is downregulated in ALL and directly represses JARID1B.
  • Dysregulated JARID1B expression due to miR-137 downregulation may contribute to ALL pathogenesis via aberrant histone methylation.
Keywords:
MiR-137adult acute lymphoblastic leukemialymphoblastic leukemia

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