Biosafety of unmodified ultrafine gold particles (AuPs) upon interacting with human blood components before systemic...

Ultrafine gold particles (AuPs) can be emerged as a good candidate in the field of drug delivery as well as in imaging applications. However, little attention has been paid to detailed study of nanoparticle's interaction with blood components before systemic use. An investigation into the interaction of ultrafine AuPs with blood components is must for its clinical application. In present study, the interaction of ultrafine sized AuPs (2 ± 0.5 nm, 5 ± 1 nm, and 10 ± 2 nm) with blood components and its immunogenic property (pro-inflammatory reaction) was investigated. All three sized AuPs did not cause any significant hemolysis. Plasma coagulation study showed significant increase in Prothrombin time (PT) with International Normalized Ratio (INR) value raised to 1.53 with 10 nm AuPs. Maximum prolongation of activated partial thromboplastin time (APTT) (3.2 s) was seen with 5 &10 nm sized AuPs. Maximum thrombin time (TT) prolongation was seen with 2 nm (18.3s) with the difference of 1.4 s as compared to control. Platelet aggregation was faster in case of 5 & 10 nm sized AuPs. All three sized AuPs exhibited in-vitro C3 complement activation whereas they did not stimulate significant proliferation of peripheral blood mononuclear cells (PBMC). These findings further validate the utility of ultrafine AuPs for in-vivo applications.