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Updated: Jan 23, 2026

Establishment of a Clonal Culture of Unicellular Conjugating Algae
Published on: July 14, 2018
New method for algae comprehensive utilization: Algae-derived biochar enhances algae anaerobic fermentation for
Xu Duan1, Yunzhi Chen2, Yuanyuan Yan3
1State Key Laboratory of Pollution Control and Resources Reuse, School of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, China.
Abstract:
Interest in the resource utilization of algae has gradually increased due to the frequent occurrence of harmful algal blooms. Here, biochar derived from algae was applied to algae anaerobic fermentation for short-chain fatty acids (SCFAs) production. In the presence of algae-derived biochar, the concentration of SCFAs within 4 d (4334 mg COD/L) was approximately doubled compared to the control (2016 mg COD/L), and the fermentation time for maximal SCFAs yield was shortened. Biochar improved the disruption of algae to release more intracellular macromolecular organics. Altering algae hydrolysis, the activity of hydrolase and the contents of functional gene were advantageous to SCFAs accumulation by providing more micromolecular organics in the presence of biochar. Additionally, the relative abundance and survival of acid-forming bacteria were enhanced significantly. Furthermore, biochar accelerated the electron transport and energy synthesis in the biological system, driving the biological reactions that allow microorganisms to function and life to flourish.
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